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A new interspecific, Gossypium hirsutum × G. barbadense, RIL population: towards a unified consensus linkage map of tetraploid cotton

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Abstract

We report the development of a new interspecific cotton recombinant inbred line (RIL) population of 140 lines deriving from an interspecific cross between Gossypium hirsutum (Gh) and G. barbadense (Gb), using the same two parents that have served for the construction of a BC1 map and for the marker-assisted backcross selection program underway at CIRAD. Two marker systems, microsatellites and AFLPs, were used. An important feature of the RIL population was its marked segregation distortion with a genome-wide bias to Gh alleles (parental genome ratio is 71/29). The RIL map displays an excellent colinearity with the BC1 map, although it is severely contracted in terms of map size. Existence of 255 loci in common (between 6 and 14 per chromosome) allowed the integration of the two data sets. A consensus BC1–RIL map based upon 215 individuals (75 BC1 + 140 RIL) was built. It consisted of 1,745 loci, spanned 3,637 cM, intermediate between the sizes of the two component maps, and constituted a solid framework to cross align cotton maps using common markers. The new RIL population will be further exploited for fiber property QTL mapping and eQTL mapping.

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Acknowledgments

The French National Research Agency, ANR, through its program specialized in plant genomics, Génoplante, has sponsored this research (project nr ANR-06-GPLA-018) that was otherwise supported by Bayer CS and CIRAD funding.

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Authors and Affiliations

  1. CIRAD, UMR DAP, Avenue Agropolis, 34398, Montpellier Cedex 5, France

    Jean-Marc Lacape, N. Forestier-Chiron, J. Jean, E. Thomas & C. Viot

  2. Bayer BioScience N.V., Technologiepark 38, Ghent, Belgium

    J. Jacobs & R. Derijcker

  3. Bayer CropScience, BioScience Research, Lubbock, TX, USA

    T. Arioli

  4. CSIRO, Plant Industry, Canberra, ACT, 2601, Australia

    D. Llewellyn

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  1. Jean-Marc Lacape
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  2. J. Jacobs
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  3. T. Arioli
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Correspondence to Jean-Marc Lacape.

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Communicated by C. Gebhardt.

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Lacape, JM., Jacobs, J., Arioli, T. et al. A new interspecific, Gossypium hirsutum × G. barbadense, RIL population: towards a unified consensus linkage map of tetraploid cotton. Theor Appl Genet 119, 281–292 (2009). https://doi.org/10.1007/s00122-009-1037-y

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  • DOI: https://doi.org/10.1007/s00122-009-1037-y

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