Abstract
The Phaseolus vulgaris I locus-Bean common mosaic virus (BCMV; Potyviridae) pathosystem is of critical importance to bean geneticists, breeders and pathologists because of the worldwide distribution of both the virus and germplasm containing this resistance gene. In order to learn more about the molecular responses characteristic of this resistance gene, a cDNA-AFLP screen was conducted on homozygous NILs of P. vulgaris variety ‘Black Turtle Soup’ (BT), containing either the I locus allele for resistance (BTII) or susceptibility (BTii) to BCMV. Eight conditions were compared in a factorial analysis: BTII versus BTii; mock inoculated versus BCMV inoculated; 26 versus 34°C. Transcripts induced in response to viral infection and that were further responsive to temperature, genotype or both were isolated and cloned. Sequence analysis of the resultant clones revealed several classes of putative genes, including transcription-related and signal transduction-related genes. Review of disease resistance literature suggests further avenues of research involving the candidates isolated in this screen.
Similar content being viewed by others
References
Bachem CWB, vanderHoeven RS, deBruijn SM, Vreugdenhil D, Zabeau M, Visser RGF (1996) Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development. Plant J 9:745–753
Belkhadir Y, Subramaniam R, Dangl JL (2004) Plant disease resistance protein signaling: NBS-LRR proteins and their partners. Curr Opin Plant Biol 7:391–399
van der Biezen EA, Jones JDG (1998) Plant disease-resistance proteins and the gene-for-gene concept. Trends Biochem Sci 23:454–456
Bos L (1971) Bean common mosaic virus. Descriptions of plant viruses, vol 73
Cadle-Davidson MM (2005) Mechanism of resistance to Bean common mosaic virus conferred by the I locus in Phaseolus vulgaris L. Dissertation. Plant breeding and genetics. Cornell University, Ithaca, p 98
Cadle-Davidson MM, Jahn MM (2005) Resistance conferred against Bean common mosaic virus by the incompletely dominant I locus of Phaseolus vulgaris is active at the single cell level. Arch Virol 150:2601–2608
Collmer CW, Marston MF, Taylor JC, Jahn M (2000) The I gene of bean: a dosage-dependent allele conferring extreme resistance, hypersensitive resistance, or spreading vascular necrosis in response to the potyvirus Bean common mosaic virus. Mol Plant Microbe Interact 13:1266–1270
Dangl JL, Jones JDG (2001) Plant pathogens and integrated defence responses to infection. Nature 411:826–833
Drijfhout E (1991) Bean common mosaic. Compendium of bean diseases. APS Press, Saint paul, pp 37–39
Ferrier-Cana E, Macadre C, Sevignac M, David P, Langin T, Geffroy V (2005) Distinct post-transcriptional modifications result into seven alternative transcripts of the CC-NBS-LRR gene JA1tr of Phaseolus vulgaris. Theor Appl Genet 110:895–905
Heath RL, McDonald G, Christeller JT, Lee M, Bateman K, West J, vanHeeswijck R, Anderson MA (1997) Proteinase inhibitors from Nicotiana alata enhance plant resistance to insect pests. J Insect Physiol 43:833–842
Hull R (2002) Matthews’ plant virology. Academic, San Diego
Jones AL (2002) G-protein-coupled signaling in Arabidopsis. Curr Opin Plant Biol 5:402–407
Jones DA, Takemoto D (2004) Plant innate immunity—direct and indirect recognition of general and specific pathogen-associated molecules. Curr Opin Immunol 16:48–62
Kachroo A, Lapchyk L, Fukushige H, Hildebrand D, Klessig DF, Kachroo P (2003) Plastidial fatty acid signaling modulates salicylic acid- and jasmonic acid-mediated defense pathways in the Arabidopsis ssi2 mutant. Plant Cell 15:2952–2965
Kaldis AD, Kousidis P, Kesanopoulos K, Prombona A (2003) Light and circadian regulation in the expression of LHY and Lhcb genes in Phaseolus vulgaris. Plant Mol Biol 52:981–997
Leung J, Merlot S, Giraudat J (1997) The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. Plant Cell 9:759–771
Maleck K, Levine A, Eulgem T, Morgan A, Schmid J, Lawton KA, Dangl JL, Dietrich RA (2000) The transcriptome of Arabidopsis thaliana during systemic acquired resistance. Nat Genet 26:403–410
Provvidenti R (1983) Two useful selections of the bean cultivar Black Turtle Soup for viral identification. Bean Improv Coop Annu Rep 26:73–75
Rathjen JP, Moffett P (2003) Early signal transduction events in specific plant disease resistance. Curr Opin Plant Biol 6:300–306
Roehl T, Caliebe A, Seedorf M, Soll J (1995) Characterization of four cDNAs encoding small GTP-binding proteins from pea. Plant Physiol 109:1125
Shah J, Kachroo P, Nandi A, Klessig DF (2001) A recessive mutation in the Arabidopsis SSI2 gene confers SA- and NPR1-independent expression of PR genes and resistance against bacterial and oomycete pathogens. Plant J 25:563–574
Urcuqui-Inchima S, Haenni A-L, Bernardi F (2001) Potyvirus proteins: a wealth of functions. Virus Res 74:157–175
Vallejos CE, Malandro JJ, Sheehy K, Zimmermann MJ (2000) Detection and cloning of expressed sequences linked to a target gene. Theor Appl Genet 101:1109–1113
Zegzouti H, Jones B, Frasse P, Marty C, Maitre B, Latche A, Pech J, Bouzayen M (1999) Ethylene-regulated gene expression in tomato fruit: characterization of novel ethylene-responsive and ripening-related genes isolated by differential display. Plant J 18:589–600
Zhang YL, Fan W, Kinkema M, Li X, Dong X (1999) Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene. Proc Natl Acad Sci USA 96:6523–6528
Zhang YL, Tessaro MJ, Lassner M, Li X (2003) Knockout analysis of Arabidopsis transcription factors TGA2, TGA5, and TGA6 reveals their redundant and essential roles in systemic acquired resistance. Plant Cell 15:2647–2653
Zhou JM, Trifa Y, Silva H, Ponteir D, Lam E, Shah J, Klessig DF (2000) NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid. Mol Plant Microbe Interact 13:191–200
Acknowledgements
We thank L. Cadle-Davidson, G. Rairdan, S. Restrepo, P. Griffiths, S. McCouch, and T. Zitter for critical review of this manuscript and insightful discussions during the course of this work and G. Moriarty for technical assistance. M.M. Cadle-Davidson was supported in part by the Interdisciplinary Research Training Group in Molecular Mechanisms of Plant Processes (NSF Grant DBI-9420687).
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by F. J. Muehlbauer
Rights and permissions
About this article
Cite this article
Cadle-Davidson, M., Jahn, M.M. Differential gene expression in Phaseolus vulgaris I locus NILs challenged with Bean common mosaic virus . Theor Appl Genet 112, 1452–1457 (2006). https://doi.org/10.1007/s00122-006-0247-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-006-0247-9