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QTL mapping of resistance to gray leaf spot in ryegrass

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Abstract

Gray leaf spot (GLS) is a serious fungal disease caused by Magnaporthe grisea, recently reported on perennial ryegrass (Lolium perenne L.), an important turfgrass and forage species. This fungus also causes rice blast and many other grass diseases. Rice blast is usually controlled by host resistance, but durability of resistance is a problem. Little GLS resistance has been reported in perennial ryegrass. However, greenhouse inoculations in our lab using one ryegrass isolate and one rice-infecting lab strain suggest presence of partial resistance. A high density linkage map of a three generation Italian × perennial ryegrass mapping population was used to identify quantitative trait loci (QTL) for GLS resistance. Potential QTL of varying effect were detected on four linkage groups, and resistance to the ryegrass isolate and the lab strain appeared to be controlled by different QTL. Of three potential QTL detected using the ryegrass isolate, the one with strongest effect for resistance was located on linkage group 3 of the MFB parent, explaining between 20% and 37% of the phenotypic variance depending on experiment. Another QTL was detected on linkage group 6 of the MFA parent, explaining between 5% and 10% of the phenotypic variance. The two QTL with strongest effect for resistance to the lab strain were located on linkage groups MFA 2 and MFB 4, each explaining about 10% of the phenotypic variance. Further, the QTL on linkage groups 3 and 4 appear syntenic to blast resistance loci in rice. This work will likely benefit users and growers of perennial ryegrass, by setting the stage for improvement of GLS resistance in perennial ryegrass through marker-assisted selection.

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Acknowledgements

We thank Drs. Mark Farman and Andy Hamblin for providing us the perennial ryegrass isolates, and Drs. Michael Havey and Michael Casler for critical reviews for the manuscript. We thank Jung lab members for their excellent technical assistance. Funding for this project was provided by the United States Golf Association and partially by the Wisconsin Turfgrass Association.

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Authors and Affiliations

  1. Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, 53706, USA

    J. Curley, S. C. Sim & G. Jung

  2. US National Arboretum, Washington, DC, 20002, USA

    S. Warnke

  3. USDA-ARS, Madison, WI, 53706, USA

    S. Leong

  4. USDA-ARS, Corvallis, OR, 97331, USA

    R. Barker

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  1. J. Curley
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  2. S. C. Sim
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  3. S. Warnke
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  4. S. Leong
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  5. R. Barker
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  6. G. Jung
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Correspondence to G. Jung.

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Communicated by T. Lübberstedt

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Curley, J., Sim, S.C., Warnke, S. et al. QTL mapping of resistance to gray leaf spot in ryegrass. Theor Appl Genet 111, 1107–1117 (2005). https://doi.org/10.1007/s00122-005-0036-x

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  • DOI: https://doi.org/10.1007/s00122-005-0036-x

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