Article

Theoretical and Applied Genetics

, Volume 107, Issue 6, pp 997-1002

Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L.

  • K. SuwabeAffiliated withNational Institute of Vegetable and Tea Science (NIVTS), 360 Kusawa, Ano, Age, Mie 514-2392, Japan
  • , H. TsukazakiAffiliated withNational Institute of Vegetable and Tea Science (NIVTS), 360 Kusawa, Ano, Age, Mie 514-2392, Japan
  • , H. IketaniAffiliated withNational Agricultural Research Organization, 3-1-1 Kannon-dai, Tsukuba, Ibaraki 305-8517, Japan
  • , K. HatakeyamaAffiliated withNational Institute of Vegetable and Tea Science (NIVTS), 360 Kusawa, Ano, Age, Mie 514-2392, Japan
  • , M. FujimuraAffiliated withNational Institute of Vegetable and Tea Science (NIVTS), 360 Kusawa, Ano, Age, Mie 514-2392, Japan
  • , T. NunomeAffiliated withNational Institute of Vegetable and Tea Science (NIVTS), 360 Kusawa, Ano, Age, Mie 514-2392, Japan
  • , H. FukuokaAffiliated withNational Institute of Vegetable and Tea Science (NIVTS), 360 Kusawa, Ano, Age, Mie 514-2392, Japan
  • , S. MatsumotoAffiliated withNational Institute of Vegetable and Tea Science (NIVTS), 360 Kusawa, Ano, Age, Mie 514-2392, Japan Email author 
  • , M. HiraiAffiliated withNational Institute of Vegetable and Tea Science (NIVTS), 360 Kusawa, Ano, Age, Mie 514-2392, JapanFaculty of Agriculture, University of Kyoto prefecture, Kitainaazuma, Seika, Kyoto 619-0244, Japan

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Abstract

In an analysis of 114 F2 individuals from a cross between clubroot-resistant and susceptible lines of Brassica rapa L., 'G004' and 'Hakusai Chukanbohon Nou 7' (A9709), respectively, we identified two loci, Crr1 and Crr2, for clubroot (caused by Plasmodiophora brassicae Woronin) resistance. Each locus segregated independently among the F2 population, indicating that the loci reside on a different region of chromosomes or on different chromosomes. Genetic analysis showed that each locus had little effect on clubroot resistance by itself, indicating that these two loci are complementary for clubroot resistance. The resistance to clubroot was much stronger when both loci were homozygous for resistant alleles than when they were heterozygous. These results indicate that clubroot resistance in B. rapa is under oligogenic control and at least two loci are necessary for resistance.

Keywords

Brassica rapa Clubroot Microsatellites (SSRs) Plasmodiophora brassicae Disease resistance