Theoretical and Applied Genetics

, Volume 104, Issue 8, pp 1290–1297

Seed gene flow and fine-scale structure in a Mediterranean pine (Pinus pinaster Ait.) using nuclear microsatellite markers

  •  S. González-Martínez
  •  S. Gerber
  •  M. Cervera
  •  J. Martínez-Zapater
  •  L. Gil
  •  R. Alía

DOI: 10.1007/s00122-002-0894-4

Cite this article as:
González-Martínez, S., Gerber, S., Cervera, M. et al. Theor Appl Genet (2002) 104: 1290. doi:10.1007/s00122-002-0894-4

Abstract.

The Mediterranean populations of maritime pine (Pinus pinaster Ait.) are typically small and have a scattered distribution, being threatened by human activities and forest fires. In the framework of the genetic-resources conservation program of this species, a native multi-age stand located in a Mediterranean area (central Spain) was studied using three highly polymorphic nuclear microsatellites (SSRs). Spatial autocorrelation analysis was conducted using Moran's index in order to detect fine-scale structure in both natural regeneration and mature trees. The spatial pattern of seed flow based on dispersed progeny was studied using a highly reliable subset of parent-offspring matches obtained by means of parentage analysis and simulation-based calculation of statistical confidence. Maritime pine showed a fine-scale structure at the seedling stage. In natural regeneration, the autocorrelograms indicated a patch size of approximately 10 m. The fine-scale structure seems to be produced by a restricted seed gene flow. In fact, there was an excess of parent-offspring matches in a radius of 15 m from the parent trees. Pines with a heavy seed, such as P. pinaster, are expected to have a short dispersal distance, thus producing a fine-scale structure. However, the fine-scale structure did not persist in the mature trees. Within-population genetic structure in Mediterranean pines may be affected by a number of post-dispersal events (e.g. mortality due to the severity of the Mediterranean climate and animal-mediated secondary dispersal during the summer period). Thus, great alteration in the pattern produced by the initial seed rain and differences in genetic structure between tree cohorts are expected.

LOD-scores SSRs Genetic conservation Pinus pinaster Iberian Peninsula

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  S. González-Martínez
    • 1
  •  S. Gerber
    • 3
  •  M. Cervera
    • 1
  •  J. Martínez-Zapater
    • 1
  •  L. Gil
    • 2
  •  R. Alía
    • 1
  1. 1.Departamento de Mejora Genética y Biotecnología, INIA, P.O. 8111, 28080 Madrid, Spain
  2. 2.Unidad de Anatomía, Fisiología y Genética, ETSIM, Ciudad Universitaria s/n, 28040 Madrid, Spain
  3. 3.Laboratoire de génétique et amélioration des arbres forestiers, INRA, BP 45, 33611 Gazinet Cedex, France
  4. 4.Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma de Madrid en Cantoblanco, 28049 Madrid, Spain