Journal of Molecular Medicine

, Volume 78, Issue 12, pp 692–702

Tumor regression induced by intratumoral injection of DNA coding for human interleukin 12 into melanoma metastases in gray horses

  • Lucie M. Heinzerling
  • Karsten Feige
  • Stefan Rieder
  • Margarete K. Akens
  • Reinhard Dummer
  • Gerald Stranzinger
  • Karin Moelling
Original Article

DOI: 10.1007/s001090000165

Cite this article as:
Heinzerling, L., Feige, K., Rieder, S. et al. J Mol Med (2001) 78: 692. doi:10.1007/s001090000165

Abstract

Preclinical studies investigating new therapeutic principles against melanoma are presently being carried out in mouse models; however, these are not optimal. Here we describe a novel animal model using gray horses. These animals spontaneously develop metastatic melanoma that resembles human disease and is thus highly relevant for preclinical studies testing new immunotherapy protocols. We found that injection of plasmid DNA coding for the human cytokine interleukin 12 into established metastases induced significant regression in all 12 treated lesions in a total of 7 horses. Complete disappearance was observed in one treated lesion, with no recurrence after 6 months. No adverse events have been observed in any of the animals during and after treatment. These results demonstrate the effectiveness and safety of interleukin 12 encoding plasmid DNA therapy against established metastatic disease in a large animal model and serve as a basis for a clinical trial.

Large animal Melanoma model Plasmid DNA treatment Species specificity Cytokine

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Lucie M. Heinzerling
    • 1
  • Karsten Feige
    • 2
  • Stefan Rieder
    • 3
  • Margarete K. Akens
    • 2
  • Reinhard Dummer
    • 4
  • Gerald Stranzinger
    • 3
  • Karin Moelling
    • 1
  1. 1.Institute of Medical Virology, University of Zurich, Gloriastrasse 30, 8028 ZurichSwitzerland
  2. 2.Department of large Animal Internal Medicine, Veterinary University Hospital Zurich, Winterthurerstrasse 260, 8057 ZurichSwitzerland
  3. 3.Department of Animal Sciences, Swiss Federal Institute of Technology, Tannenstrasse 1, 8092 ZurichSwitzerland
  4. 4.Department of Dermatology, University of Zurich Medical School, Gloriastrasse 31, 8091 ZurichSwitzerland