Abstract
Objective
The objective of this in vitro study was an initial screening of the cytotoxic potential of widely used smooth enamel surface sealants.
Materials and methods
A total of 20 products were allocated to four groups based on their chemical composition: (1) filled resin-based sealants, (2) unfilled resin-based sealants, (3) a resin-modified, glass ionomer-based sealant, and (4) silicone-based sealants. All materials were applied to human enamel slices both in accordance with manufacturers’ instructions and in additional experiments applying 50 % undercuring and 50 % overcuring. An agar overlay assay was then used to test the specimens following ISO 10933. The cytotoxic potential of each material was interpreted based on a reaction index that summarized the decolorization and lysis scores obtained.
Results
The cytotoxic potential decreased as follows: unfilled resin-based sealants > filled resin-based sealants > resin-modified, glass ionomer-based sealant > silicone-based sealants. In 75 % of the resin-based products, deliberate undercuring was associated with more extensive decolorization zones, leading to higher rates of cytotoxic potential in two of those products. Overcuring, by contrast, was associated with a tendency for smaller decolorization zones in 50 % of the resin-based products.
Conclusion
Surface sealants derived from resin monomers exhibited cytotoxic potential in the agar overlay assay. There is also evidence of a possible association with curing, as undercuring can increase the cytotoxic potential, whereas normal curing (as per manufacturers’ instructions) or overcuring may help minimize such effects. More research into the biological implications of these materials is needed, especially with regard to their potential impact on the adjacent gingiva.
Zusammenfassung
Ziel
Ziel dieser In-vitro-Studie war, das zytotoxische Potenzial gängiger Versiegelungsmaterialien für Glattflächen im Sinne eines „initialen Screenings“ zu untersuchen.
Material und Methodik
Insgesamt 20 Produkte wurden nach ihren chemischen Eigenschaften in 4 Gruppen eingeteilt: 1) kompositbasiert, gefüllt; 2) kompositbasiert, ungefüllt, 3) kunststoffmodifiziert glasionomerbasiert und 4) silikonbasiert. Die Materialien wurden gemäß Herstellerangaben sowie mit 50 % verkürzter und verlängerter Polymerisations- bzw. Trocknungszeit auf humane Schmelzscheiben aufgetragen und im Agar-Overlay-Assay in Anlehnung an ISO 10933 getestet. Für die Interpretation wurden Reaktionsindizes auf der Basis von Entfärbungs- und Lyseindizes erstellt.
Ergebnisse
Das zytotoxische Potenzial nahm in der folgenden Reihenfolge der Untersuchungsgruppen ab: kompositbasiert, ungefüllt > kompositbasiert, gefüllt > kunststoffmodifiziert glasionomerbasiert > silikonbasiert. Bei verkürzter Polymerisationszeit zeigten 75 % der kompositbasierten Produkte erhöhte Entfärbungszonen; bei 2 Produkten war dies mit einer erhöhten Einstufung des zytotoxischen Potenzials verbunden. Bei entsprechender Verlängerung der Polymerisationszeit konnte bei 50 % der kompositbasierten Produkte eine tendenzielle Reduzierung der Entfärbungszonen beobachtet werden.
Schlussfolgerungen
Versiegelungsmaterialien für Glattflächen auf Basis von Kunststoffmonomeren weisen im Agar-Overlay-Assay zytotoxisches Potenzial auf. Ferner zeichnet sich ein möglicher Zusammenhang zwischen Polymerisationszeit und zytotoxischem Potenzial ab. Reduzierte Polymerisationszeiten können das zytotoxische Potenzial erhöhen. Umgekehrt kann die Einhaltung bzw. Verlängerung der Polymerisationszeiten helfen, das zytotoxische Potenzial zu reduzieren. Weitere Studien, insbesondere zur möglichen Beeinflussung der benachbarten Gingiva, sind notwendig, um die biologischen Effekte dieser Materialien weiter bewerten zu können.
Abbreviations
- bis-GMA:
-
bisphenol-A diglycidyl dimethacrylate
- HEMA:
-
hydroxyethyl methacrylate
- HPLC:
-
high performance liquid chromatography
- MTT:
-
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide
- TEGDMA:
-
triethylene glycol dimethacrylate
- UDMA:
-
urethane dimethacrylate
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Acknowledgements
The authors would like to thank the German Orthodontic Society (Deutsche Gesellschaft für Kieferorthopädie) for providing financial support for this project.
Compliance with ethical guidelines
Conflict of interest. S. Zingler, B. Matthei, A. Kohl, D. Saure,B. Ludwig, K. Diercke, C.J. Lux, and R. Erber state that there are no conflicts of interest. The accompanying manuscript does not include studies on humans or animals.
Danksagung
Die Autoren danken der Deutschen Gesellschaft für Kieferorthopädie (DGKFO) für die finanzielle Unterstützung des Projektes aus dem Wissenschaftsfond.
Einhaltung ethischer Richtlinien
Interessenkonflikt. S. Zingler, B. Matthei, A. Kohl, D. Saure,B. Ludwig, K. Diercke, C.J. Lux, R. Erber geben an, dass kein Interessenkonflikt besteht. Dieser Beitrag beinhaltet keine Studien an Menschen oder Tieren.
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Zingler, S., Matthei, B., Kohl, A. et al. In vitro studies on the cytotoxic potential of surface sealants. J Orofac Orthop 76, 66–78 (2015). https://doi.org/10.1007/s00056-014-0269-x
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DOI: https://doi.org/10.1007/s00056-014-0269-x