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Resorption behavior of a nanostructured bone substitute: in vitro investigation and clinical application

Untersuchung zum Resorptionsverhalten eines nanostrukturierten Knochenaufbaumaterials. In-vitro-Untersuchungen und klinische Anwendung

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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie Aims and scope Submit manuscript

Abstract

Objectives

To develop an in vitro assay for quantitative analysis of the degradation to which a bone substitute is exposed by osteoclasts. The aim of establishing this method was to improve the predictability of carrying out tooth movements via bone substitutes and to provide a basis for verification in exemplary clinical cases.

Methods

After populating a bone substitute (NanoBone®; ArtOss, Germany) with osteoclastic cells, inductively-coupled mass spectrometry was used to evaluate changing calcium levels in the culture medium as a marker of resorption activity.

Results

It was observed that calcium levels increased substantially in the culture medium with the cells populating the bone substitute.

Conclusions

This in vitro assay is a valid method that can assist clinicians in selecting the appropriate materials for certain patients. While tooth movements occurring through this material were successful, uncertainty about the approach will remain as long-term results are not available.

Zusammenfassung

Zielsetzung

Das Ziel der Untersuchung war die Entwicklung eines In-vitro-Ansatzes zur Quantifizierung der Degradation eines Knochenersatzmaterials durch Osteoklasten. Auf Basis dieser Untersuchungen sollte die Zahnbewegung durch Knochenersatzmaterialien vorhersagbarer werden und in klinischen Beispielen überprüft werden.

Methode

Für die In-vitro-Untersuchung wurde ein Knochenersatzmaterial (NanoBone®, ArtOss, Deutschland) mit osteoklastären Zellen besiedelt und die Veränderungen der Kalziumkonzentration im Kulturmedium als Marker der Resorptionsaktiität mittels induktiver Massenspektrometrie gemessen.

Ergebnisse

In der in-vitro-Untersuchung zeigte sich über den Untersuchungszeitraum nach Besiedelung des Knochenersatzmaterials mit den Zellen eine deutliche Zunahme des Kalziumgehaltes im Kulturmedium.

Schlussfolgerung

Die hier beschriebene In-vitro-Methode ist ein valider Ansatz, die dem Behandler helfen kann die richtige Materialauswahl für seinen spezifischen Patienten zu treffen. Die Zahnbewegungen durch das hier beschrieben Material war erfolgreich, jedoch bleiben immer noch einige Unsicherheiten, da Langzeitergebnisse fehlen.

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Conflict of interest

On behalf of all authors, the corresponding author states the following: CR and WG are consultants for ArtOss.

Interessenkonflikt

Der korrespondierende Autor weist für sich und seine Koautoren auf folgende Beziehung/en hin: CR und WG sind als Referenten der Fa. ArtOss tätig.

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Authors and Affiliations

  1. Department for Orthodontics, Rheinische Friedrich-Wilhelm University of Bonn, Welschnonnenstr. 17, 53111, Bonn, Germany

    C. Reichert, W. Götz, S. Reimann & A. Jäger

  2. Endowed Professorship for Oral Medical Technology and Laboratory for Experimental Orthodontics, University of Bonn, Bonn, Germany

    S. Reimann, L. Keilig & C. Bourauel

  3. Private Practice for Periodontology, Bonn, Germany

    M. Hagner

Authors
  1. C. Reichert
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  2. W. Götz
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  3. S. Reimann
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  4. L. Keilig
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  5. M. Hagner
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  6. C. Bourauel
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  7. A. Jäger
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Corresponding author

Correspondence to C. Reichert.

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Reichert, C., Götz, W., Reimann, S. et al. Resorption behavior of a nanostructured bone substitute: in vitro investigation and clinical application. J Orofac Orthop 74, 165–175 (2013). https://doi.org/10.1007/s00056-012-0136-6

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  • DOI: https://doi.org/10.1007/s00056-012-0136-6

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