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Dimethyl disulfide and dimethyl trisulfide: so similar yet so different in evoking biological responses in saprophilous flies

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Abstract

Dimethyl disulfide (DMDS) and dimethyl trisulfide (DMTS) are used by saprophilous insects to locate breeding sites (decaying organic matter), and by brood-site deceptive flowers to attract such insects. However, little is known about the relative importance of these two compounds in eliciting electrophysiological and behavioral responses in the insects. Here, we compared the relative attractiveness of DMDS and DMTS to saprophilous flies in field choice experiments and tested whether potential differences in field responses can be explained by differences in electrophysiological antennal responses to these compounds. Field experiments revealed that the attractiveness of a mixture of these compounds is due to DMTS alone. This result was confirmed by electroantennographic recordings in which flies of four tested species of Calliphoridae (Lucilia sericata, L. caesar, Calliphora vicina, Protocalliphora azurea) and one Muscidae (Musca domestica) respond clearly to DMTS, but not to DMDS. In house flies, however, DMTS elicited electrophysiological responses only, not reflected in behavioral assays. Despite the fact that DMTS and DMDS exhibit similar chemical structures, both the electroantennographic and field responses from saprophilous flies to these two compounds strongly differed. Our study suggests that oligosulfide-responsive saprophilous flies rely on DMTS and not DMDS for finding appropriate breeding sites and that DMTS and not DMDS could act as a key mediator for pollinator attraction in brood-site deceptive plants.

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References

  • Aak A, Knudsen GK, Soleng A (2010) Wind tunnel behavioural response and field trapping of the blowfly Calliphora vicina. Med Vet Entomol 24:250–257

    CAS  PubMed  Google Scholar 

  • Archer MS, Elgar MA (2003) Effects of decomposition on carcass attendance in a guild of carrion-breeding flies. Med Vet Entomol 17:263–271

    Article  CAS  PubMed  Google Scholar 

  • Cossé AA, Baker TC (1996) House flies and pig manure volatiles: wind tunnel behavioral studies and electrophysiological evaluations. J Agric Entomol 13:301–317

    Google Scholar 

  • Dötterl S, Füssel U, Jürgens A, Aas G (2005) 1,4-Dimethoxybenzene, a floral scent compound in willows that attracts an oligolectic bee. J Chem Ecol 31:2993–2998

    Article  PubMed  Google Scholar 

  • Frederickx C, Dekeirsschieter J, Verheggen FJ, Haubruge E (2012) Responses of Lucilia sericata Meigen (Diptera: Calliphoridae) to cadaveric volatile organic compounds. J Forensic Sci 57:386–390

    Article  PubMed  Google Scholar 

  • Jhumur US, Dötterl S, Jürgens A (2007) Electrophysiological and behavioural responses of mosquitoes to volatiles of Silene otites (Caryophyllaceae). Arthropod Plant Interact 1:245–254

    Article  Google Scholar 

  • Jürgens A, Dötterl S, Meve U (2006) The chemical nature of fetid floral odours in stapeliads (Apocynaceae–Asclepiadoideae–Ceropegieae). New Phytol 172:452–468

    Article  PubMed  Google Scholar 

  • Jürgens A, Wee SL, Shuttleworth A, Johnson SD (2013) Chemical mimicry of insect oviposition sites: a global analysis of convergence in angiosperms. Ecol Lett 16:1157–1167

    Article  PubMed  Google Scholar 

  • Kalinová B, Podskalská H, Růzicka J, Hoskovec M (2009) Irresistible bouquet of death—how are burying beetles (Coleoptera: Silphidae: Nicrophorus) attracted by carcasses. Naturwissenschaften 96:889–899

    Article  PubMed  Google Scholar 

  • Kite GC, Hetterscheid WLA, Lewis MJ, Boyce PC, Ollerton J, Cocklin E, Diaz A, Simmonds MSJ (1998) Inflorescence odours and pollinators of Arum and Amorphophallus (Araceae). In: Owens SJ, Rudall PJ (eds) Reproductive Biology. Royal Botanical Gardens, Kew, pp 295–315

    Google Scholar 

  • Lam K, Tsang M, Labrie A, Gries R, Gries G (2010) Semiochemical mediated oviposition avoidance by female house flies, Musca domestica, on animal feces colonized with harmful fungi. J Chem Ecol 36:141–147

    Article  CAS  PubMed  Google Scholar 

  • Moré M, Cocucci AA, Raguso RA (2013) The importance of oligosulfides in the attraction of fly pollinators to the brood-site deceptive species Jaborosa rotacea (Solanaceae). Int J Plant Sci 174:863–876

    Article  Google Scholar 

  • Nilssen AC, Tømmerås BÅ, Schmid R, Evensen SB (1996) Dimethyl trisulphide is a strong attractant for some calliphorids and a muscid but not for the reindeer oestrids Hypoderma tarandi and Cephenemyia trompe. Entomol Exp Appl 79:211–218

    Article  CAS  Google Scholar 

  • Paczkowski S, Maibaum F, Paczkowska M, Schütz S (2012) Decaying mouse volatiles perceived by Calliphora vicina Rob.-Desv. J Forensic Sci 57:1497–1506

    Article  PubMed  Google Scholar 

  • Park KC, Cork A (1999) Electrophysiological responses of antennal receptor neurons in female Australian sheep blowflies, Lucilia cuprina, to host odours. J Insect Physiol 45:85–91

    Article  CAS  PubMed  Google Scholar 

  • Schwab W, Davidovich-Rikanati R, Lewinsohn E (2008) Biosynthesis of plant-derived flavor compounds. Plant J 54:712–732

    Article  CAS  PubMed  Google Scholar 

  • Shuttleworth A, Johnson SD (2010) The missing stink: sulphur compounds can mediate a shift between fly and wasp pollination systems. Proc Roy Soc Lond B Biol Sci 277:2811–2819

    Article  CAS  Google Scholar 

  • Stensmyr MC, Urru I, Collu I, Celander M, Hansson BS, Angioy A (2002) Rotting smell of dead-horse arum florets. Nature 420:625–626

    Article  CAS  PubMed  Google Scholar 

  • Urru I, Stensmyr MC, Hansson BS (2011) Pollination by brood-site deception. Phytochemistry 72:1655–1666

    Article  CAS  PubMed  Google Scholar 

  • van der Niet T, Hansen DM, Johnson SD (2011) Carrion mimicry in a South African orchid: flowers attract a narrow subset of the fly assemblage on animal carcasses. Ann Bot 107:981–992

    Article  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

We would like to thank Dr. Irmgard Schäffler for methodological support and constructive discussions, two anonymous reviewers and Dr. Artur Maia for their helpful comments on an earlier version of the manuscript, and the owners of the pigsty for agreeing to perform the experiments at their property.

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Correspondence to Stefan Dötterl.

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Handling Editor: Michael Heethoff.

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Zito, P., Sajeva, M., Raspi, A. et al. Dimethyl disulfide and dimethyl trisulfide: so similar yet so different in evoking biological responses in saprophilous flies. Chemoecology 24, 261–267 (2014). https://doi.org/10.1007/s00049-014-0169-y

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  • DOI: https://doi.org/10.1007/s00049-014-0169-y

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