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Effect of metabolites isolated from Cuscuta pedicellata on high fat diet-fed rats

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Abstract

The aim of the present study was to determine if Cuscuta pedicellata extract has an effect on body weight and serum lipid profile in the HFD-fed rats’ animal model. The ethanol extract of C. pedicellata exhibited a significant reduction in body weight and serum lipid profile in this model. The n-hexane/EtOAc (1:1), EtOAc and MeOH/EtOAc (1:1) fractions were also active, while the MeOH fraction showed no beneficial effect. Bioactivity-guided fractionation leads to the isolation of ten pure compounds: naringenin (1), kaempferol (2), aromadenderin (3), quercitin (4), 3,5,7,3′,5′-pentahydroxy flavanone (5), naringenin -7-O-β-d-glucoside (6), aromadenderin -7-O-β-d-glucoside (7), Taxifolin -7-O-β-d-glucoside (8), kaempferol -3-O-β-d-glucoside (astragalin), (9) and quercitin -3-O-β-d-glucoside (isoquercitrin) (10). This is the first report of compounds 1, 3, 5, 6, 7, and 8 in Cuscuta pedicellata. Compound 1 > compound 8 > compound 3 > compound 7 > compound 4 > compound 2 are responsible for the potency of the ethanol extract, while compound 6 was only effective in reducing the total cholesterol serum levels but not effective in reducing the level of triglycerides or the body weight. Our data confirmed the anti-obesity effect of C. pedicellata reported by the Egyptian population and identified the compounds responsible for this activity.

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Acknowledgments

The authors are grateful to Prof. Dr. Abd Elraof A., Professor of Botany, Faculty of Science, Suez Canal University for taxonomical identification of the plant.

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Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmacy, Sinai University, North Sinai, 55441, Egypt

    Salwa H. Zekry & Rawia A. Zayed

  2. Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt

    Dina M. Abo-elmatty

  3. Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig, 44519, Egypt

    Rawia A. Zayed

  4. National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA

    Mohamed M. Radwan

  5. Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt

    Mohamed M. Radwan & Mahmoud A. ElSohly

  6. Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA

    Mahmoud A. ElSohly

  7. Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt

    Hashim A. Hassanean & Safwat A. Ahmed

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  1. Salwa H. Zekry
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  2. Dina M. Abo-elmatty
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  3. Rawia A. Zayed
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Correspondence to Safwat A. Ahmed.

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Zekry, S.H., Abo-elmatty, D.M., Zayed, R.A. et al. Effect of metabolites isolated from Cuscuta pedicellata on high fat diet-fed rats. Med Chem Res 24, 1964–1973 (2015). https://doi.org/10.1007/s00044-014-1269-5

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  • DOI: https://doi.org/10.1007/s00044-014-1269-5

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