Medicinal Chemistry Research

, Volume 23, Issue 3, pp 1225–1233

Cytotoxic activity assessment and c-Src tyrosine kinase docking simulation of thieno[2,3-b] pyridine-based derivatives

Authors

  • Ali Reza Nikkhoo
    • Department of ToxicologyAJA University of Medical Science
    • Medicinal & Natural Products Chemistry Research CenterShiraz University of Medical Sciences
    • Department of Medicinal Chemistry, School of PharmacyShiraz University of Medical Sciences
  • Ramin Miri
    • Medicinal & Natural Products Chemistry Research CenterShiraz University of Medical Sciences
    • Department of Medicinal Chemistry, School of PharmacyShiraz University of Medical Sciences
  • Nahid Arianpour
    • Department of ToxicologyAJA University of Medical Science
  • Omidreza Firuzi
    • Medicinal & Natural Products Chemistry Research CenterShiraz University of Medical Sciences
  • Ahmad Ebadi
    • Medicinal & Natural Products Chemistry Research CenterShiraz University of Medical Sciences
    • Department of Medicinal Chemistry, School of PharmacyShiraz University of Medical Sciences
    • Department of ToxicologyAJA University of Medical Science
Original Research

DOI: 10.1007/s00044-013-0729-7

Cite this article as:
Nikkhoo, A.R., Miri, R., Arianpour, N. et al. Med Chem Res (2014) 23: 1225. doi:10.1007/s00044-013-0729-7

Abstract

Thienopyridine derivatives possess various promising biological properties and particularly cytotoxic effect. In vitro cytotoxic activities of some thienopyridine analogous were evaluated by MTT reduction assay in three human cancer cell lines (HL-60, MCF-7, and LS-180). The compounds showed a wide range of cytotoxic activities and their IC50 values ranged from 0.2 to 100 μM and above. Compound 4e was the most potent derivative and 4i showed good cytotoxic activity against all three cell lines (IC50 <20 μM). Docking simulation of thienopyridine derivatives was implemented on c-Src tyrosine kinase involved in tumor progression and metastases. Results showed that these compounds might potentially bind to the key amino acid Thr339 in the c-Src tyrosine kinase active site. Ligand efficiency (LE) values calculated by using free binding energies obtained from experimental data were predicted by the docking study. Also, experimental and predicted LEs were in good agreement. Based on the LE indices and other findings, some of the thienopyridine derivatives might be efficient candidates for further development as anticancer agents.

Keywords

ThienopyridineCytotoxic activityDocking simulationLigand efficiency

Copyright information

© Springer Science+Business Media New York 2013