Research Article

Cellular and Molecular Life Sciences

, Volume 64, Issue 3, pp 365-376

First online:

Identification of steroid hormone signaling pathway in insect cell differentiation

  • D. SiaussatAffiliated withUMR 1272 Physiologie de l’Insecte, Signalisation et Communication, Université Pierre et Marie Curie
  • , F. BozzolanAffiliated withUMR 1272 Physiologie de l’Insecte, Signalisation et Communication, Université Pierre et Marie Curie
  • , P. PorcheronAffiliated withUMR 1272 Physiologie de l’Insecte, Signalisation et Communication, Université Pierre et Marie Curie
  • , S. DebernardAffiliated withUMR 1272 Physiologie de l’Insecte, Signalisation et Communication, Université Pierre et Marie Curie Email author 

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Abstract.

To dissect the steroid hormone signaling pathway involved in insect cell morphological differentiation, we extended the application of the doublestranded RNA-mediated interference (dsRNAi) method to the epidermal IAL-PID2 cell line from Plodia interpunctella Lepidoptera. We first demonstrated that dsRNA was capable of efficiently blocking the steroid hormone 20-hydroxyecdysone (20E) inducibility of proteins that belong to the nuclear receptor superfamily, including the ecdysone receptor (EcR), its partner Ultraspiracle (USP), the insect homolog of the vertebrate retinoid X receptor and the HR3 transcription factor. We then showed that inhibiting the 20E induction of EcR, USP or HR3 proteins prevented the increased synthesis of β tubulin and consequently the morphological transformation of cells. Thanks to this functional approach, we have shown, for the first time, the participation of EcR, USP and HR3 in a 20E signaling pathway that directs morphological differentiation in insect cells by regulating β tubulin expression.

Keywords.

Steroid hormone receptor superfamily cell morphological differentiation RNA interference β tubulin subnit signaling pathway