Article

Inflammation Research

, Volume 47, Issue 4, pp 167-173

Interleukin-13 increases prostaglandin E2 (PGE2) production by normal human polymorphonuclear neutrophils by enhancing cyclooxygenase 2 (COX-2) gene expression

  • C.-L. YuAffiliated withDepartment of Medicine and Institute of Clinical Medicine, Veterans General Hospital-Taipei, No. 201, Section 2, Shih-Pai Road, Taipei, Taiwan, Fax +886 2 2871 3241
  • , M.-H. HuangAffiliated withDepartment of Medicine and Institute of Clinical Medicine, Veterans General Hospital-Taipei, No. 201, Section 2, Shih-Pai Road, Taipei, Taiwan, Fax +886 2 2871 3241
  • , Y.-Y. KungAffiliated withDepartment of Medicine and Institute of Clinical Medicine, Veterans General Hospital-Taipei, No. 201, Section 2, Shih-Pai Road, Taipei, Taiwan, Fax +886 2 2871 3241
  • , C.-Y. TsaiAffiliated withDepartment of Medicine and Institute of Clinical Medicine, Veterans General Hospital-Taipei, No. 201, Section 2, Shih-Pai Road, Taipei, Taiwan, Fax +886 2 2871 3241
  • , Y.-Y. TsaiAffiliated withDepartment of Medicine and Institute of Clinical Medicine, Veterans General Hospital-Taipei, No. 201, Section 2, Shih-Pai Road, Taipei, Taiwan, Fax +886 2 2871 3241
  • , S.-T. TsaiAffiliated withDepartment of Family Medicine, Veterans General Hospital-Taipei, Taipei, Taiwan
  • , D.-F. HuangAffiliated withDepartment of Medicine and Institute of Clinical Medicine, Veterans General Hospital-Taipei, No. 201, Section 2, Shih-Pai Road, Taipei, Taiwan, Fax +886 2 2871 3241
  • , K.-H. SunAffiliated withFaculty of Medical Technology, National Yang-Ming University, Taipei, Taiwan
  • , S.-H. HanAffiliated withDepartment of Medicine and Institute of Clinical Medicine, Veterans General Hospital-Taipei, No. 201, Section 2, Shih-Pai Road, Taipei, Taiwan, Fax +886 2 2871 3241
    • , H.-S. YuAffiliated withDepartment of Dermatology, Kaohsiung Medical College, Kaohsiung, Taiwan

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Abstract

Objective: To investigate whether interleukin-13 (IL-13) can affect arachidonic acid metabolism and phagocytic activity of normal human polymorphonuclear neutrophils (PMN).¶Methods: Normal human PMN (1 × 106 cells/ml) were incubated with different concentrations of IL-13 (0.1–10 ng/ml) for a variety of times (30–120 min). Phagocytosis and intracellular cyclooxygenase-2 (COX-2) were detected by flow cytometry. The expression of COX-1 and COX-2 mRNA was detected by RT-PCR. The concentration of PGE2 in the PMN cultured supernatants was determined by EIA.¶Results: We found that IL-13 at an optimal concentration of 1 ng/ml significantly enhanced COX-2 gene expression and PGE2 production (121.57 ± 22.17 pg/ml in IL-13 stimulation vs. 73.16 ± 11.72 pg/ml in controls) by PMN. In addition, IL-13 stimulated PMN phagocytosis via increased complement receptor type 1 (CR1) and type 3 (CR3), but not IgG Fcγ receptor type 3 (FcγRIII). The cytoplasmic neutral esterase activity of PMN was also enhanced by IL-13 stimulation for 24 h.¶Conclusions: These results suggest that IL-13 can stimulate PMN and modulates the inflammatory reactions via the cyclooxygenase pathway.

Key words: Interleukin 13 — Polymorphonuclear neutrophil — Prostaglandin E2— Cyclooxygenase 2 — Neutral esterase