Inflammation Research

, Volume 47, Issue 4, pp 167–173

Interleukin-13 increases prostaglandin E2 (PGE2) production by normal human polymorphonuclear neutrophils by enhancing cyclooxygenase 2 (COX-2) gene expression

  • C.-L. Yu
  • M.-H. Huang
  • Y.-Y. Kung
  • C.-Y. Tsai
  • Y.-Y. Tsai
  • S.-T. Tsai
  • D.-F. Huang
  • K.-H. Sun
  • S.-H. Han
  • H.-S. Yu
Article

DOI: 10.1007/s000110050312

Cite this article as:
Yu, C., Huang, M., Kung, Y. et al. Inflamm. res. (1998) 47: 167. doi:10.1007/s000110050312

Abstract

Objective: To investigate whether interleukin-13 (IL-13) can affect arachidonic acid metabolism and phagocytic activity of normal human polymorphonuclear neutrophils (PMN).¶Methods: Normal human PMN (1 × 106 cells/ml) were incubated with different concentrations of IL-13 (0.1–10 ng/ml) for a variety of times (30–120 min). Phagocytosis and intracellular cyclooxygenase-2 (COX-2) were detected by flow cytometry. The expression of COX-1 and COX-2 mRNA was detected by RT-PCR. The concentration of PGE2 in the PMN cultured supernatants was determined by EIA.¶Results: We found that IL-13 at an optimal concentration of 1 ng/ml significantly enhanced COX-2 gene expression and PGE2 production (121.57 ± 22.17 pg/ml in IL-13 stimulation vs. 73.16 ± 11.72 pg/ml in controls) by PMN. In addition, IL-13 stimulated PMN phagocytosis via increased complement receptor type 1 (CR1) and type 3 (CR3), but not IgG Fcγ receptor type 3 (FcγRIII). The cytoplasmic neutral esterase activity of PMN was also enhanced by IL-13 stimulation for 24 h.¶Conclusions: These results suggest that IL-13 can stimulate PMN and modulates the inflammatory reactions via the cyclooxygenase pathway.

Key words: Interleukin 13 — Polymorphonuclear neutrophil — Prostaglandin E2— Cyclooxygenase 2 — Neutral esterase

Copyright information

© Birkhäuser Verlag, Basel, 1998

Authors and Affiliations

  • C.-L. Yu
    • 1
  • M.-H. Huang
    • 1
  • Y.-Y. Kung
    • 1
  • C.-Y. Tsai
    • 1
  • Y.-Y. Tsai
    • 1
  • S.-T. Tsai
    • 2
  • D.-F. Huang
    • 1
  • K.-H. Sun
    • 3
  • S.-H. Han
    • 1
  • H.-S. Yu
    • 4
  1. 1.Department of Medicine and Institute of Clinical Medicine, Veterans General Hospital-Taipei, No. 201, Section 2, Shih-Pai Road, Taipei, Taiwan, Fax +886 2 2871 3241TW
  2. 2.Department of Family Medicine, Veterans General Hospital-Taipei, Taipei, TaiwanTW
  3. 3.Faculty of Medical Technology, National Yang-Ming University, Taipei, TaiwanTW
  4. 4.Department of Dermatology, Kaohsiung Medical College, Kaohsiung, TaiwanTW