Skip to main content
SpringerLink
Log in

Distinct gene expression profiles provoked by polyacrylamide beads (Biogel) during chronic and acute inflammation in mice selected for maximal and minimal inflammatory responses

  • Original Research Paper
  • Published:
Inflammation Research Aims and scope Submit manuscript

Abstract

Objective and design

AIRmax and AIRmin mice differ in their local acute inflammatory reactions to polyacrylamide beads (Biogel). These lines were developed to identify genes that affect the intensity of the acute inflammatory response (AIR) and to investigate the cellular and molecular mechanisms of acute inflammation. Although these lines are well established, differences in their responses to chronic inflammatory Biogel exposure have not yet been described. We investigated whether the selective process that modified the acute inflammatory responses in these animals also affected the development of their chronic inflammatory responses.

Results

Inflammatory exudate cell infiltration was more intense in AIRmax than AIRmin mice at both 48 h and 30 days. Genes involved in signal transduction and immune/inflammatory responses were differentially expressed in the treated skin of AIRmax and AIRmin mice, and divergent expression of some acute inflammatory response genes was detected up to 30 days post-Biogel. However, distinct expression of several pro and anti-inflammatory response genes in both periods was observed.

Conclusion

These results indicate that the selective process for acute inflammation affected the development of chronic inflammatory responses to Biogel, suggesting common genetic control.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Abbreviations

AIR:

Acute Inflammatory Response

CCL2:

Chemokine (C–C motif) ligand 2

CXCL1:

Chemokine (C–X–C motif) ligand 1

CXCL2:

Chemokine (C–X–C motif) ligand 2

CXCR2:

Chemokine (C–X–C motif) receptor 2

DAMP:

Danger-associated molecular pattern

EASE:

Expression Analysis Systematic Explorer

FDR:

False Discovery Rate

IL-1β:

Interleukin-1 beta

IL-6:

Interleukin-6

PAMP:

Pathogen-associated molecular pattern

PCR:

Polymerase chain reaction

PRR:

Pattern recognition receptor

QTL:

Quantitative Trait Loci

SAM:

Significance Analysis of Microarray

SNP:

Single Nucleotide Polymorphism

TGF-β:

Transforming growth factor, beta 1

TNF-α:

Tumor necrosis factor alpha

References

  1. Medzhitov R. Origin and physiological roles of inflammation. Nature. 2008;13:428–35.

    Article  Google Scholar 

  2. Perreti M. Endogenous mediators that inhibit the leukocyte–endothelium interaction. Trends Pharmacol Sci. 1997;18:418–25.

    Google Scholar 

  3. Stiffel C, Ibañez OM, Ribeiro OG, Decreusenfond C, Mouton D, Siqueira M, Biozzi G. Genetics of acute inflammation: inflammatory reaction in inbred lines of mice and their interline crosses. Exp Clin Immunogenet. 1990;7:221–33.

    CAS  PubMed  Google Scholar 

  4. Ibañez OM, Stiffel C, Ribeiro OG, Cabrera WH, Massa S, De Franco M, Sant`ana OA, Decreusenfond C, Mouton D, Siqueira M, Biozzi G. Genetics of nonspecific immunity: I. Bidirectional selective breeding of lines of mice endowed with maximal or minimal inflammatory responsiveness. Eur J Immunol. 1992;22:2555–63.

    Article  PubMed  Google Scholar 

  5. Biozzi G, Ribeiro OG, Saran A, Araújo ML, Maria DA, De Franco M, Cabrera WH, Sant`anna OA, Massa S, Covelli V, Mouton D, Neveu T, Siqueira M, Ibañez OM. Effect of genetic modification of acute inflammatory responsiveness on tumorigenesis in the mouse. Carcinogenesis. 1998;18:337–46.

    Article  Google Scholar 

  6. Araújo LM, Ribeiro OG, Siqueira M, De Franco M, Starobinas N, Massa S, Cabrera WH, Mouton D, Seman M, Ibañez OM. Innate resistance to infection by intracellular bacterial pathogens differs in mice selected for maximal or minimal acute inflammatory response. Eur J Immunol. 1998;28:2913–20.

    Article  PubMed  Google Scholar 

  7. Vigar ND, Cabrera WH, Araújo LM, Ribeiro OG, Ogata TR, Siqueira M, Ibañez OM, De Franco M. Pristane-induced arthritis in mice selected for maximal or minimal acute inflammatory reaction. Eur J Immunol. 2000;30:431–7.

    Article  CAS  PubMed  Google Scholar 

  8. Vorraro F, Galvan A, Cabrera WHK, Carneiro PS, Ribeiro OG, De Franco M, Starobinas N, Jensen JR, Seman M, Dragani TA, Ibañez OCM. Genetic control of IL-1b production and inflammatory response by the mouse Irm1 locus. J Immunol. 2010;2010(185):1616–21.

    Article  Google Scholar 

  9. Galvan A, Vorraro F, Cabrera W, Ribeiro OG, Starobinas N, Jensen JR, Carneiro PS, De Franco M, Gao X, Ibañez OCM, Dragani TA. Association study by genetic clustering detects multiple inflammatory response loci in non-inbred mice. Genes Immun. 2011;12:390–4.

    Article  CAS  PubMed  Google Scholar 

  10. Giulietti A, Overbergh L, Valckx D, Decallonne B, Bouillon R, Mathieu C. An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. Methods. 2001;25:386–440.

    Article  CAS  PubMed  Google Scholar 

  11. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods. 2001;25:402–8.

    Article  CAS  PubMed  Google Scholar 

  12. Kobayashi Y. Neutrophil infiltration and chemokines. Crit Rev Immunol. 2006;26:307–16.

    Article  CAS  PubMed  Google Scholar 

  13. Ribeiro OG, Maria DA, Adriouch S, Pechberty S, Cabrera WHK, Morriset J, Ibañez OM, Seman M. Convergent alteration of granulopoiesis, chemotactic activity, and neutrophil apoptosis during mouse selection for high acute inflammatory response. J Leukoc Biol. 2003;74:497–506.

    Article  CAS  PubMed  Google Scholar 

  14. Carneiro OS, Peters LC, Vorraro F, Borrego A, Ribeiro OG, Starobinas N, Jensen JR, Cabrera WHK, Ibañez OM, De Franco M. Gene expression profiles of bone marrow cells from mice phenotype-selected for maximal or minimal acute inflammations: searching for genes in acute inflammation modifier loci. Immunology. 2009;2009(128):562–71.

    Article  Google Scholar 

  15. De Franco M, Peters LC, Correa MA, Galvan A, Canhamero T, Borrego A, Jensen JR, Gonçalves J, Cabrera WH, Starobinas N, Ribeiro OG, Dragani TA, Ibañez OM. Pristane-induced arthritis loci interact with the Slc11a1 gene to determine susceptibility in mice selected for high inflammation. PLoS One. 2014;9(2):e88302.

    Article  PubMed  PubMed Central  Google Scholar 

  16. Leemans JC, Cassel SL, Sutterwala FS. Sensing damage by the NLRP3 inflammasome. Immunol Rev. 2011;243:152–62.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Prince LR, Whyte MK, Sabroe I, Parker LC. The role of TLRs in neutrophil activation. Cur Opin Pharmacol. 2011;11:397–403.

    Article  CAS  Google Scholar 

  18. Chen GY, Nuñez G. Sterile inflammation: sensing and reacting to damage. Nat Rev Immunol. 2010;10:827–37.

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by FAPESP and CNPq. MDF and OMI have fellowship from CNPq.

Author information

Authors and Affiliations

  1. Laboratório de Imunogenética, Instituto Butantan, Avenida Vital Brasil 1500, São Paulo, SP, 05503900, Brazil

    Jussara Gonçalves Fernandes, Tatiane Canhamero, Andrea Borrego, José Ricardo Jensen, Wafa Hanna Cabrera, Mara Adriana Correa, Nancy Starobinas, Orlando Garcia Ribeiro, Olga Martinez Ibañez & Marcelo De Franco

Authors
  1. Jussara Gonçalves Fernandes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tatiane Canhamero
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andrea Borrego
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. José Ricardo Jensen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wafa Hanna Cabrera
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mara Adriana Correa
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Nancy Starobinas
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Orlando Garcia Ribeiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Olga Martinez Ibañez
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Marcelo De Franco
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marcelo De Franco.

Ethics declarations

Conflict of interest

No competing interests.

Additional information

Responsible Editor: John Di Battista.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fernandes, J.G., Canhamero, T., Borrego, A. et al. Distinct gene expression profiles provoked by polyacrylamide beads (Biogel) during chronic and acute inflammation in mice selected for maximal and minimal inflammatory responses. Inflamm. Res. 65, 313–323 (2016). https://doi.org/10.1007/s00011-016-0918-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00011-016-0918-1

Keywords

Search

Navigation