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Polysaccharides of Dendrobium officinale inhibit TNF-α-induced apoptosis in A-253 cell line

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Abstract

Objective

Our previous study demonstrated that polysaccharides of Dendrobium officinale Kimura et Migo (DP) were capable of enhancing immunomodulation in an experimental model of Sjögren’s syndrome, a chronic autoimmune disease mainly affecting the salivary glands. In the present study, we further investigated the protective effect of DP on a human salivary gland cell line A-253 against tumor necrosis factor (TNF)-α-induced apoptosis.

Materials

TNF-α (100 U/ml) was used as the stimulus for treating the A-253 cells to induce cellular apoptosis. Nuclear factor-kappa B (NF-κB, p65), phosphorylation of mitogen-activated protein kinases (MAPK), reactive oxygen species (ROS) generation, mitochondrial membrane potential and proapoptotic proteins were examined. A-253 cells were pre-treated with DP for 12 h before TNF-α stimulation.

Results

We observed translocation of NF-κB into the nuclei, prolonged MAPK, excessive ROS generation and strongly decreased mitochondrial membrane potential, and subsequently cytochrome C release and caspase-3 activation. However, pre-treatment with DP significantly inhibited the TNF-α-induced apoptotic factors.

Conclusions

Our data suggested the inhibitory effect of DP on TNF-α-induced apoptosis in a human salivary gland cell line. This inhibition indicated potential inference of DP in the initial plasma membrane-bound complex of TNF-α and its receptors.

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Acknowledgments

This work was supported by grants (Project No. 20730472 and Project No. 201011159206) from the University of Hong Kong.

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Authors and Affiliations

  1. School of Chinese Medicine, The University of Hong Kong, 1/F, Estate Building, 10 Sassoon Road, Pokfulam, Hong Kong, China

    L. Xiang, C. W. Stephen Sze, Y. Tong & Y. B. Kalin Zhang

  2. School of Biomedicine Science, Shatin, TN, Hong Kong, China

    T. B. Ng

  3. School of Life Science, Shatin, TN, Hong Kong, China

    P. C. Shaw

  4. Department of Medicine, Faculty of Medicine, The University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong, China

    C. W. Sydney Tang

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  1. L. Xiang
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  2. C. W. Stephen Sze
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Correspondence to Y. B. Kalin Zhang.

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Responsible Editor: John Di Battista.

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Xiang, L., Stephen Sze, C.W., Ng, T.B. et al. Polysaccharides of Dendrobium officinale inhibit TNF-α-induced apoptosis in A-253 cell line. Inflamm. Res. 62, 313–324 (2013). https://doi.org/10.1007/s00011-012-0584-x

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  • DOI: https://doi.org/10.1007/s00011-012-0584-x

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