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Non-invasive screening of progressive joint defects in the Type II collagen-induced arthritis animal model using radiographic paw images

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Abstract

Objective

This study presents a method for non-invasive screening of progressive arthritic reactions in a representative rheumatoid arthritis (RA) animal model of Type II collagen-induced arthritis (CIA).

Materials

Seventeen male DBA/1J mice (9 weeks old) were used for the control group (seven) and the CIA group (ten).

Treatment

The CIA model was induced by the injection of a Type II collagen and incomplete Freund’s adjuvant emulsion.

Methods

Arthritic reactions including joint space narrowing (JSN), bone erosion, inflammation, and physiological defects were observed using five non-invasive methods. The degrees of JSN and bone erosion were quantified using macro-radiographs of specific joints and bones, while the severity of inflammation was evaluated by changes in paw volume and visual scoring of edema and erythema.

Results

Significant differences were observed in JSN (≤48.29%, p < 0.0001), bone erosion (78.30%, p < 0.0001), grip strength (p < 0.05), and inflammation (91.67% in edema and 194.12% increase in paw volume, p < 0.001) between the CIA group and the controls. The relationships between factors exhibited significant positive correlations (R ≥ 0.9229, p < 0.05) between radiographic parameters, with symmetries of ≥0.9062 (p < 0.05) for JSN and ≥ 0.8575 (p < 0.001) for bone erosion, 0.8770 (p < 0.0001) for inflammatory parameters, and ≥0.9527 (p < 0.0001) for radiographic and non-radiographic parameters.

Conclusions

Our non-invasive radiographic and non-radiographic methods of documentation were both acceptable for screening joint defects during RA progression. In addition, the results of this study may be useful for monitoring the progression of joint pathology in RA diseases.

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References

  1. Choi S, Lee YA, Hong SJ, Lee GJ, Kang SW, Park JH, Park JH, Park HK. Evaluation of changes in inflammation and bony erosion on type II collagen-induced arthritis animal model. Rheumatol Int. 2010. [Epub ahead of print].

  2. Lehmann TM, Beier D, Thies C, Seidl T. Segmentation of medical images combining local, regional, global, and hierarchical distances into a bottom-up region merging scheme. Medical imaging 2005: Image processing. In: Fitzpatrick JM, Reinhardt JM, editors. Proceedings of the SPIE, 2005. vol 5747. p. 546–55.

  3. Anatomical homology. http://ncse.com/book/export/html/863. Accessed 6 July 2009.

  4. Yu HL, Strauch B, Chase RA. Atlas of hand anatomy and clinical implications. St. Louis: Mosby; 2004.

    Google Scholar 

  5. American society for surgery of the hand, anatomy of the hand. http://www.eatonhand.com/hom/hom033.htm, E-Hand Textbook Table of Contents. Accessed 6 July 2009.

  6. Bielecki A, Korkosz M, Zieliński B. Hand radiographs preprocessing, image representation in the finger regions and joint space width measurements for image interpretation. Pattern Recogn. 2008;41:3786–98.

    Article  Google Scholar 

  7. Buckland-Wright JC, Walker SR. Incidence and size of erosions in the wrist and hand of rheumatoid patients: a quantitative microfocal radiographic study. Ann Rheum Dis. 1987;46:463–7.

    Article  PubMed  CAS  Google Scholar 

  8. Brook A, Corbett M. Radiographic changes in early rheumatoid disease. Ann Rheum Dis. 1977;36:71–3.

    Article  PubMed  CAS  Google Scholar 

  9. Courtenay JS, Dallman MJ, Dayan AD, Martin A, Mosedale B. Immunization against heterologous type II collagen induces arthritis in mice. Nature. 1980;283:666–8.

    Article  PubMed  CAS  Google Scholar 

  10. Khurana R, Berney SM. Clinical aspects of rheumatoid arthritis. Pathophysiology. 2005;12:153–65.

    Article  PubMed  Google Scholar 

  11. Tan Sardjono C, Mottram PL, van de Velde NC, Powell MS, Power D, Slocombe RF, Wicks IP, Campbell IK, McKenzie SE, Brooks M, Stevenson AW, Hogarth PM. Development of spontaneous multisystem autoimmune disease and hypersensitivity to antibody-induced inflammation in Fc gamma receptor IIa-transgenic mice. Arthritis Rheum. 2005;52:3220–9.

    Article  PubMed  Google Scholar 

  12. Ory PA. Interpreting radiographic data in rheumatoid arthritis. Ann Rheum Dis. 2003;62:597–604.

    Article  PubMed  CAS  Google Scholar 

  13. Brand DD, Latham KA, Rosloniec EF. Collagen-induced arthritis. Nat Protoc. 2007;2:1269–75.

    Article  PubMed  CAS  Google Scholar 

  14. Chronic Disease Prevention, Public Health Branch, Rural & Regional Health & Aged Care Services Division of the Victorian State Government, Department of Human Services, Australia, Arthritis & musculoskeletal disorders. http://www.health.vic.gov.au/nhpa/arthritis.htm. National Health Priority Areas background paper. Accessed 13 Jun 2006.

  15. Lubberts E, Joosten LA, Van Den Bersselaar L, Helsen MM, Bakker AC, Xing Z, Richards CD, Van Den Berg WB. Intra-articular IL-10 gene transfer regulated the expression of collagen-induced arthritis (CIA) in the knee and ipsilateral paw. Clin Exp Rheumatol. 2000;120:375–83.

    CAS  Google Scholar 

  16. Rheumatoid arthritis treatment. http://www.hopkins-arthritis.org/arthritis-info/rheumatoid-arthritis/rheum_treat.html, the Johns Hopkins Arthritis Center. Accessed 14 July 2009.

  17. Klippel JH. Primer on the rheumatic diseases. In: Klippel JH, Stone JH, Crofford LEJ, White PH, editors. 12th ed. Arthritis foundation, Atlanta; 1998.

  18. National Rheumatoid Arthritis Society Website. http://www.rheumatoid.org.uk/, NRAS. Accessed 14 July 2009.

  19. Jalava S, Reunanen K. Healing of erosions in rheumatoid arthritis. Scand J Rheumatol. 1982;11:97–100.

    Article  PubMed  CAS  Google Scholar 

  20. Rau R, Wassenberg S, Herborn G, Perschel WT, Freitag G. Identification of radiologic healing phenomena in patients with rheumatoid arthritis. J Rheumatol. 2001;28:2608–15.

    PubMed  CAS  Google Scholar 

  21. Menninger H, Meixner C, Sondgen W. Progression and repair in radiographs of hands and forefeet in early rheumatoid arthritis. J Rheumatol. 1995;22:1048–54.

    PubMed  CAS  Google Scholar 

  22. Lindqvist E, Jonsson K, Saxne T, Eberhardt K. Course of radiographic damage over 10 years in a cohort with early rheumatoid arthritis. Ann Rheum Dis. 2003;62:611–6.

    Article  PubMed  CAS  Google Scholar 

  23. Bielecki A, Korkosz M, Zieliński B. Hand radiographs preprocessing, image representation in the finger regions and joint space width measurements for image interpretation. Pattern Recog. 2008;41:3786–98.

    Article  Google Scholar 

  24. Zanggera P, Keystoneb EC, Bogochc ER. Asymmetry of small joint involvement in rheumatoid arthritis: prevalence and tendency towards symmetry over time. Joint Bone Spine. 2005;72:241–7.

    Article  Google Scholar 

  25. Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, Healey LA, Kaplan SR, Liang MH, Luthra HS, Medsger Jr. TA, Mitchell DM, Neustadt DH, Pinals RS, Schaller JG, Sharp JT, Wilder RL, Hunder GG. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 1998;31:315–24.

  26. Fleming A, Crown JM, Corbett M. Early rheumatoid disease. I Onset Ann Rheum Dis. 1976;35:357–60.

    Article  CAS  Google Scholar 

  27. Resnick D, Niwayama G. Rheumatoid arthritis. In: Resnick D, editor. Diagnosis of bone and joint disorders. 3rd ed ed. Philadelphia: WB Saunders Co; 1995. p. 886.

    Google Scholar 

  28. Short CL, Bauer W. The course of rheumatoid arthritis in patients receiving simple medical and orthopaedic measures. N Engl J Med. 1948;238:142–8.

    Article  PubMed  CAS  Google Scholar 

  29. Harris ED. Clinical features of rheumatoid arthritis. In: Kelly WN, Harris ED, Ruddy S, Sledge CB, editors. Textbook of rheumatology. 4th ed. Philadelphia: WB Saunders Co; 1993. p. 876–7.

    Google Scholar 

  30. Soila P. A roentgenological study of asymmetry in rheumatoid arthritis. A preliminary communication. Acta Rheumatol Scand. 1963;9:264–8.

    Article  PubMed  CAS  Google Scholar 

  31. Wolfe F, Sharp JT. Radiographic outcome of recent-onset rheumatoid arthritis. Arthritis Rheum. 1963;41:1571–82.

    Article  Google Scholar 

  32. Boonsaner K, Louthrenoo W, Meyer S, Schumacher HR. Effect of dominancy on severity in rheumatoid arthritis. Br J Rheumatol. 1992;31:77–80.

    Article  PubMed  CAS  Google Scholar 

  33. Leak RS, Rayan GM, Arthur RE. Longitudinal radiographic analysis of rheumatoid arthritis in the hand and wrist. J Hand Surg Am. 2003;28A:427–34.

    Article  Google Scholar 

  34. Mattingly PC, Matheson JA, Dickson RA. The distribution of radiological joint damage in the rheumatoid hand. Rheumatol Rehabil. 1979;18:142–7.

    Article  PubMed  CAS  Google Scholar 

  35. Mody GM, Meyers OL, Reinach SG. Handedness and deformities, radiographic changes, and function of the hand in rheumatoid arthritis. Ann Rheum Dis. 1989;48:104–7.

    Article  PubMed  CAS  Google Scholar 

  36. Owsianik WDJ, Kundi A, Whitehead JN, Kraag GR, Goldsmith C. Radiological articular involvement in the dominant hand in rheumatoid arthritis. Ann Rheum Dis. 1980;39:508–10.

    Article  PubMed  CAS  Google Scholar 

  37. Burns TM, Calin A. The hand radiograph as a diagnostic discriminant between seropositive and seronegative ‘rheumatoid arthritis’: a controlled study. Ann Rheum Dis. 1983;42:605–12.

    Article  PubMed  CAS  Google Scholar 

  38. El-Khoury GY, Larson RK, Kathol MH, Berbaum KS, Furst DE. Seronegative and seropositive rheumatoid arthritis: radiographic differences. Radiology. 1988;168:517–20.

    PubMed  CAS  Google Scholar 

  39. Panayi GS, Celinska E, Emery P, Griffin J, Welsh KI, Grahame R, Gibson T. Seronegative and seropositive rheumatoid arthritis: similar diseases. Br J Rheumatol. 1987;26:172–80.

    Article  PubMed  CAS  Google Scholar 

  40. Bukhari M, Lunt M, Harrison BJ, Scott DGI, Symmons DPM, Silman AJ. Erosions in inflammatory polyarthritis are symmetrical regardless of rheumatoid factor status: results from a primary care-based inception cohort of patients. Rheumatology. 2002;41:246–52.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This study was supported by a research fund from Seoul R&BD (grant # CR070054).

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Authors and Affiliations

  1. Department of Biomedical Engineering & Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea

    Samjin Choi, Su-Jin Chae, Sung Wook Kang, Youjin Cheong & Hun-Kuk Park

  2. Department of Pharmacology, College of Medicine, Kyung Hee University, Seoul, 130-701, Republic of Korea

    Sung Wook Kang

  3. Division of Rheumatology, Department of Internal Medicine, College of Medicine, Kyung Hee University Medical Center, Seoul, 130-702, Republic of Korea

    Seung-Jae Hong

  4. Program of Medical Engineering, Kyung Hee University, Seoul, 130-701, Republic of Korea

    Hun-Kuk Park

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  1. Samjin Choi
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Correspondence to Hun-Kuk Park.

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Responsible Editor: John Di Battista.

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Choi, S., Chae, SJ., Kang, S.W. et al. Non-invasive screening of progressive joint defects in the Type II collagen-induced arthritis animal model using radiographic paw images. Inflamm. Res. 60, 447–456 (2011). https://doi.org/10.1007/s00011-010-0287-0

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  • DOI: https://doi.org/10.1007/s00011-010-0287-0

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