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Bacterial Infections and Osteoclastogenesis Regulators in Men and Women with Cholesteatoma

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Archivum Immunologiae et Therapiae Experimentalis Aims and scope

Abstract

One of the most distinct features of middle ear cholesteatoma is bone destruction. Aetiology of cholesteatoma is thought to be multifactorial. Endotoxins produced by bacteria are thought to initiate the inflammation process in the middle ear leading to cholesteatoma. There are physiological differences in bone metabolism between men and women. The aim of our study was the immunohistochemical evaluation of the contents of two key components of the OPG/RANK/RANKL triad—RANKL and OPG in cholesteatoma, to analyse if there are any differences between the sexes and to evaluate the bacteria species isolated from cholesteatoma just before surgical treatment and to evaluate their plausible influence on the expression of OPG and RANKL in cholesteatoma. Twenty-one adult patients with acquired cholesteatoma who underwent surgery were analysed. There were no statistically significant differences in the expression of both regulators of osteoclastogenesis between the sexes. In 38.1 % patients cholesteatoma was not infected, whereas in 61.9 % patients various bacterial infections or mycosis were found. The most frequently isolated species was Pseudomonas aeruginosa (14.29 % infections) followed by Staphylococcus aureus (9.52 % infections). There were no statistically significant differences in expression of both OPG and RANKL between uninfected and infected cholesteatomas.

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Acknowledgments

The study was financed by a grant KNW-1-096/P/2/0 from Medical University of Silesia in Katowice, Poland.

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Authors and Affiliations

  1. Department of Human Anatomy, School of Medicine, Medical University of Silesia, 18 Medyków Str., Bldg. C-1, 40-752, Katowice, Poland

    Wirginia Likus

  2. Department of Biochemistry, School of Medicine, Medical University of Silesia, Katowice, Poland

    Krzysztof Siemianowicz

  3. ENT Department, School of Medicine, Medical University of Silesia, Katowice, Poland

    Jarosław Markowski

  4. Center for Experimental Medicine, Medical University of Silesia, Katowice, Poland

    Jan Wiaderkiewicz

  5. Department of Pharmacology and Physiology, George Washington University, Washington, DC, USA

    Jan Wiaderkiewicz

  6. Department of Pathomorphology, School of Medicine, Medical University of Silesia, Katowice, Poland

    Anna Kostrząb-Zdebel

  7. Clinical Department of Internal Diseases, Allergology and Clinical Immunology, Medical University of Silesia, Katowice, Poland

    Edyta Jura-Szołtys

  8. Outpatient Clinic for Treatment of Chronic Pain, Sosnowiec, Poland

    Włodzimierz Dziubdziela

  9. Department of Histology, School of Medicine, Medical University of Silesia, Katowice, Poland

    Ryszard Wiaderkiewicz

  10. Department of Clinical and Experimental Medicine, Division of Cell Biology Integrative Regenerative Medicine Center (IGEN), Linköping University, Cell Biology Building, Level 10, 581 85, Linköping, Sweden

    Marek J. Łos

  11. Department of Pathology, Pomeranian Medical University, Szczecin, Poland

    Marek J. Łos

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  1. Wirginia Likus
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  2. Krzysztof Siemianowicz
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  3. Jarosław Markowski
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  4. Jan Wiaderkiewicz
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  5. Anna Kostrząb-Zdebel
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  6. Edyta Jura-Szołtys
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  7. Włodzimierz Dziubdziela
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  8. Ryszard Wiaderkiewicz
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  9. Marek J. Łos
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Corresponding authors

Correspondence to Wirginia Likus or Marek J. Łos.

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Likus, W., Siemianowicz, K., Markowski, J. et al. Bacterial Infections and Osteoclastogenesis Regulators in Men and Women with Cholesteatoma. Arch. Immunol. Ther. Exp. 64, 241–247 (2016). https://doi.org/10.1007/s00005-015-0373-7

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  • DOI: https://doi.org/10.1007/s00005-015-0373-7

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