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Polyphenolic extract of Termitomyces heimii: antioxidant activity and phytochemical constituents

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Abstract

Polyphenol rich fraction of Termitomyces heimii, a wild edible mushroom, was prepared to investigate its antioxidant activity and presence of different bioactive constituents. A preliminary chemical investigation was performed and it revealed that the fraction possessed various types of components with maximum amount of phenol. The other found components were in the following consequence flavonoid > ascorbic acid > ß-carotene > lycopene. HPLC data analyses showed presence of cinnamic acid and a good amount of pyrogallol (9603 µg/g of dry tissue of mushroom). The extract showed best hydroxyl radical scavenging activity (EC50 = 0.021 mg/ml). EC50 values for SOD radical scavenging assay was as low as 0.19 mg/ml. That of the rest of the antioxidant assays i.e., DPPH radical scavenging assay and reducing assay were only 0.49 and 1.31 mg/ml respectively. Thus T. heimii can be used as an easily accessible source of natural antioxidants or for the development of a new health medicine for fighting against various human stress related diseases.

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Acknowledgments

The author PM is grateful and acknowledges Innovation in Science Pursuit for Inspired Research of Department of Science and Technology for financial support.

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Authors and Affiliations

  1. Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, West Bengal, 700019, India

    Payel Mitra & Krishnendu Acharya

  2. Mycology and Plant Pathology Laboratory, Department of Botany, Visva Bharati, Santiniketan, 731235, India

    Payel Mitra & Narayan Chandra Mandal

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  1. Payel Mitra
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  2. Narayan Chandra Mandal
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  3. Krishnendu Acharya
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Correspondence to Krishnendu Acharya.

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Mitra, P., Mandal, N.C. & Acharya, K. Polyphenolic extract of Termitomyces heimii: antioxidant activity and phytochemical constituents. J. Verbr. Lebensm. 11, 25–31 (2016). https://doi.org/10.1007/s00003-015-0976-2

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  • DOI: https://doi.org/10.1007/s00003-015-0976-2

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