Abstract
Metallic nanoparticles have received considerable attention in bioassays and diagnostics due to their unique surface plasmon resonance (SPR) properties. Gold nanoparticles have been employed for the development of SPR-based colorimetric bioassays. In the present report we have described a sensitive colorimetric approach for estimation of proteins, within a detection limit of 10∼80 μg/mL, using unmodified silver nanoparticles. Besides the common advantages of colorimetric assay such as simplicity, high sensitivity, and low cost, our method has a label-free design and provides an important and attractive alternative to classical sensing probes and systems. The present work will contribute to the development of nanotechnology-based diagnostic tools.
Article PDF
Similar content being viewed by others
References
R. Elghanian, J. J. Storhoff, R. C. Mucic, R. L. Letsinger and C. A. Mirkin, Science 277, 1078 (1997). doi:10.1126/science.277.5329.1078
H. Li and L. Rothberg, Proc. Natl. Acad. Sci. USA 101, 14036 (2004). doi:10.1073/pnas.0406115101
H. Li and L. Rothberg, J. Am. Chem. Soc. 126, 10958 (2004). doi:10.1021/ja048749n
M. Mascini, G. G. Guilbaut and S. J. Lebrun, Anal. Lett. 40, 1386 (2007). doi:10.1080/00032710701327062
C. C. Huang, Y. F. Huang, Z. Cao, W. Tan and H. T. Chang, Anal. Chem. 77, 5735 (2005). doi:10.1021/ac050957q
J. S. Lee, P. A. Ulmann, M. S. Han and C. A. Mirkin, Nano Lett. 8, 529 (2008). doi:10.1021/nl0727563
Z. Wang, R. Lévy, D. G. Fernig and M. Brust, J. Am. Chem. Soc. 128, 2214 (2006). doi:10.1021/ja058135y
H. Wei, C. Chen, B. Han and Wang, Anal. Chem. 80, 7051 (2008). doi:10.1021/ac801144t
Liu and Y. Lu, J. Am. Chem. Soc. 125, 6642 (2003). doi:10.1021/ja034775u
Y. Lu and J. Liu, Acc. Chem. Res. 40, 315 (2007). doi:10.1021/ar600053g
S. K. Singh, S. Shrivastava, M. K. Nayak, A. S. K. Sinha, M. V. Jagannadham and D. Dash, J. Bionanosci. 3, 88 (2009).
S. Shrivastava, T. Bera, A. Roy, G. Singh, P. Ramachandrarao and D. Dash, Nanotechnology 18, 225103 (2007). doi:10.1088/0957-4484/18/22/225103
S. Shrivastava, T. Bera, S. K. Singh, G. Singh, P. Ramachandrarao and D. Dash, ACS Nano 3, 1357 (2009). doi:10.1021/nn900277t
Y. Chen, J. Aveyard and R. Wilson, Chem. Commun. 24, 2804 (2004). doi:10.1039/b411181h
S. Liu, Z. Zhang and M. Han, Anal. Chem. 77, 2595 (2005). doi:10.1021/ac0482864
K. Yoosaf, B. I. Ipe, C. H. Suresh and K. G. Thomas, J. Phys. Chem. C 111, 12839 (2007). doi:10.1021/jp073923q
C. L. Schofield, A. H. Haines, R. A. Field and D. A. Russell, Langmuir 22, 6707 (2006). doi:10.1021/la060288r
P. Pandey, S. P. Singh, S. K. Arya, V. Gupta, M. Datta, S. Singh and B. D. Malhotra, Langmuir 23, 3333 (2007). doi:10.1021/la062901c
D. C. Carter and J. X. Ho, Adv. Protein Chem. 45, 153 (1994). doi:10.1016/S0065-3233(08)60640-3
S. Stamler, D. J. Singel and J. Loscalzo, Science 258, 1898 (1992). doi:10.1126/science.1281928
J. Wang, L. Wang, X. Liu, Z. Liang, S. Song, W. Li, G. Li and C. Fan, Adv. Mater. 19, 3943 (2007). doi:10.1002/adma.200602256
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Shrivastava, S., Dash, D. Label-free colorimetric estimation of proteins using nanoparticles of silver. Nano-Micro Lett. 2, 164–168 (2010). https://doi.org/10.1007/BF03353636
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03353636