Immobilization effects on contractile properties of aging rat skeletal muscle

Abstract

The effect of four weeks of ankle immobilization on muscle mass and in situ contractile properties of soleus (SOL), plantaris (PLA), and extensor digitorum longus (EDL) of 31- and 37-monthold (mo) Fisher 344/Brown Norway rats was examined. Following contractile tests, muscles were harvested, weighed, and analyzed for fiber type and fiber cross-sectional area. Body weights (g) were similar in both control (CON) groups (31 mo, 578±20; 37 mo, 553±26; mean±SE) and both immobilized (IM) groups (31 mo, 496±6; 37 mo, 461±15). Immobilization-related differences in peak tetanic tension (g) were less in 37 mo than 31 mo rats (age 3treatment, p <0.05) for SOL (31 mo, CON 156±11, IM 63±12; 37 mo, CON 70±6, IM 46±8), PLA (31 mo, CON 435±13, IM 239±40; 37 mo, CON 155±14, IM 152±20) and EDL (31 mo, CON 227±13, IM 139±17; 37 mo, CON 117±16, IM 108±4). Immobilization-related differences in muscle mass (mg) were smaller in 37 mo rats compared to 31 mo animals for SOL (31 mo, 206±14 vs 129±8, 37 mo, 148±5 vs 114±2, age 3 treatment p<0.06) and PLA (31 mo, 409±14 vs 257±22, 37 mo, 234±17 vs 181±18, age 3 treatment p<0.05), but immobilization-related muscle mass differences were similar in both age groups for EDL (31 mo, 178±7 vs 134±9; 37 mo, 157±10 vs 112±7). There were no immobilization-related changes in fiber type distribution in any of the three muscles studied in either age group. The results suggest that disuse-related change is diminished when superimposed on muscles that have already undergone marked age-related decline.