Isolation and Characterization of a Barley Chitinase Genomic Clone—Expression in Powdery Mildew Infected Barley
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Chitinases (E.C.220.127.116.11) are thought to play an important role in the defense of plants against fungal invasion. By screening a barley genomic library with a previously identified chitinase eDNA clone (clone 10), a genomic clone was isolated and characterized by DNA sequencing of the chitinase coding region and flanking sequences. This clone contains an open reading frame capable of coding for a 34 kD chitinase. Comparison of the amino acid sequence of the encoded protein with other barley chitinases suggests that the genomic clone encodes chitinase T, which has been characterized extensively by protein sequencing. Treatment of barley leaves and aleurone protoplasts with N-acetyl glucosamine oligomers which act as elicitors in other plants, did not lead to the elevation of the levels of the chitinases. However, infection of barley seedlings with the powdery mildew fungus, Erysiphe graminis, resulted in the induction of several isoforms of chitinase. The level and number of chitinase isozymes was correlated with the severity of infection. The infection-related chitinases found in barley leaves are different from those found in seeds.
- Boller T, In Cellular and molecular biology of plant stress (JL Key, TS Kosuge, Editors), New York Alan R. Liss Inc. (1985) pp 247–262.
- Ignatius SMJ, Chopra RK & Muthukrishnan S, Physiol Plant, 90 (1994) 584. CrossRef
- Boller T, Gheri A, Mauch F & Vogeli U, Planta, 157 (1983) 22. CrossRef
- Mauch F, Hadwiger LA & Boller T, Plant Physiol, 76 (1984) 607. CrossRef
- Vogeli U, Meins F & Boller T, Planta, 174 (1988) 364. CrossRef
- Kragh KM, Jacobsen S & Mikkelsen JD, Plant Science, 71 (1990) 55. CrossRef
- Leah R, Tommerup H, Svendsen I & Mundy J, J Bioi Chem, 266 (1991) 1564.
- Swegle M, Barley seed chitinases, Dissertation, Kansas State University, USA (1991).
- Jacobsen S, Mikkelsen JD & Hejgaard J, Physiol Plant, 79 (1990) 554. CrossRef
- Swegle M, Huang JK, Lee G & Muthukrishnan S, Plant Mol Bioi, 12 (1989) 403. CrossRef
- Maniatis T, Fritsch EF & Sambrook J, Molecular cloning: A laboratory manual, Cold Spring Harbour Laboratory, Cold Spring Harbour, New York (1982).
- Sanger S, Nicklen S & Coulson AR, Proc Nat! Acad Sci, USA, 74 (1977) 5463. CrossRef
- Gopalakrishnan B, Sonthayanon B, Rahmatullah R & Muthukrishnan S, Plant Mol Bioi, 16 (1991) 463. CrossRef
- Von Heijne HG, Nucl Acids Res, 14 (1986) 4683. CrossRef
- Ryan CA, Biochem, 27 (1988) 8879. CrossRef
- Shinshi H, Neuhaus JM, Ryals J & Meins Jr F, Plant Mol Bioi, 14 (1990) 357. CrossRef
- Stanford A, Bevan M & Northcote O, Mol Gen Genet, 215 (1989) 200. CrossRef
- Payne G, Ahl P, Moyer M, Harper A, Beck J, Meins Jr F & Ryals J, Proc Nat! Acad Sci, USA, 87 (1990) 98. CrossRef
- Metraux JP, Burkhart W, Moyer M, Dincher S, Middlesteadt W, Williams S, Payne G, Carnes M & Ryals J, Proc Natl Acad Sci, USA, 86 (1988) 896.
- Shinshi H, Wenzler H, Neuhaus JM, Felix G, Hofsteenge J & Meins Jr F, Proc Natl Acad Sci, USA, 85 (1988) 5541. CrossRef
- Dombrowski JE, Schroeder MR, Bendnarek SY & Reikhel NV, Plant Cell, 5 (1993) 587.
- Zhu Q & Lamb CJ, Mol Gen Genet, 226 (1991) 289. CrossRef
- Isolation and Characterization of a Barley Chitinase Genomic Clone—Expression in Powdery Mildew Infected Barley
Journal of Plant Biochemistry and Biotechnology
Volume 3, Issue 2 , pp 91-95
- Cover Date
- Print ISSN
- Online ISSN
- Springer India
- Additional Links
- genomic clone
- Erysiphe graminis