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Genetic Diversity Analysis of Elite Pearl Millet Inbred Lines using RAPD and SSR Markers

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Abstract

Pearl millet [Pennisetum glaucum (L) R Br] is one of the widely grown cereal crops in the arid and semi-arid regions of Africa and India. We undertook a study to ascertain the genetic diversity in 21 elite inbreds (parental lines of 13 pearl millet hybrids in India) using 20 Random Amplified Polymorphic DNA (RAPD) and 21 Simple Sequence Repeat (SSR) markers. Based on Polymorphism Information Content (PIC) and unique banding profiles, 6 RAPD primers OPD12, OPA16, OPB6, OPA19, OPB5 and OPB1, and 3 SSR markers Xpsmp2208, Xpsmp2223 and Xpsmp2220, were found to be highly discriminative. The PIC values ranged from 0.28 to 0.48 for the RAPD and from 0.24 to 0.60 for the SSR markers. Cluster analysis and principal component analysis of the combined dataset of RAPD and SSR markers indicated moderate genetic divergence among the elite pearl millet germplasm, besides unraveling the genetic relationships among the male sterile lines and the restorers.

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Abbreviations

PCA:

Principal Component Analysis

PCR:

Polymerase Chain Reaction

GS:

Genetic Similarity

PIC:

Polymorphism Information Content

RAPD:

Random Amplified Polymorphic DNA

SSR:

Simple Sequence Repeat

UPGMA:

Unweighted Pair Group Method using Arithmetic Averages

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Author notes

  1. A. C. Chandra-Shekara

    Present address: Department of Plant Pathology, University of Kentucky, Lexington, USA

Authors and Affiliations

  1. Division of Genetics, Indian Agricultural Research Institute, New Delhi, 110 012, India

    A. C. Chandra-Shekara, B. M. Prasanna & B. B. Singh

  2. National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    S. R. Bhat

Authors
  1. A. C. Chandra-Shekara
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  2. B. M. Prasanna
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  3. S. R. Bhat
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  4. B. B. Singh
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Corresponding author

Correspondence to B. M. Prasanna.

Additional information

Part of the PhD thesis submitted to the Post-Graduate School, Indian Agricultural Research Institute, New Delhi, India

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Chandra-Shekara, A.C., Prasanna, B.M., Bhat, S.R. et al. Genetic Diversity Analysis of Elite Pearl Millet Inbred Lines using RAPD and SSR Markers. J. Plant Biochem. Biotechnol. 16, 23–28 (2007). https://doi.org/10.1007/BF03321924

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  • DOI: https://doi.org/10.1007/BF03321924

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