Journal of Plant Biochemistry and Biotechnology

, Volume 16, Issue 1, pp 23-28

First online:

Genetic Diversity Analysis of Elite Pearl Millet Inbred Lines using RAPD and SSR Markers

  • A. C. Chandra-ShekaraAffiliated withDivision of Genetics, Indian Agricultural Research Institute
  • , B. M. PrasannaAffiliated withDivision of Genetics, Indian Agricultural Research Institute Email author 
  • , S. R. BhatAffiliated withNational Research Center on Plant Biotechnology, Indian Agricultural Research Institute
  • , B. B. SinghAffiliated withDivision of Genetics, Indian Agricultural Research Institute

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Pearl millet [Pennisetum glaucum (L) R Br] is one of the widely grown cereal crops in the arid and semi-arid regions of Africa and India. We undertook a study to ascertain the genetic diversity in 21 elite inbreds (parental lines of 13 pearl millet hybrids in India) using 20 Random Amplified Polymorphic DNA (RAPD) and 21 Simple Sequence Repeat (SSR) markers. Based on Polymorphism Information Content (PIC) and unique banding profiles, 6 RAPD primers OPD12, OPA16, OPB6, OPA19, OPB5 and OPB1, and 3 SSR markers Xpsmp2208, Xpsmp2223 and Xpsmp2220, were found to be highly discriminative. The PIC values ranged from 0.28 to 0.48 for the RAPD and from 0.24 to 0.60 for the SSR markers. Cluster analysis and principal component analysis of the combined dataset of RAPD and SSR markers indicated moderate genetic divergence among the elite pearl millet germplasm, besides unraveling the genetic relationships among the male sterile lines and the restorers.

Key words

DNA fingerprinting genetic diversity Pennisetum glaucum RAPD SSR