Journal of Plant Biochemistry and Biotechnology

, Volume 16, Issue 1, pp 23–28

Genetic Diversity Analysis of Elite Pearl Millet Inbred Lines using RAPD and SSR Markers

  • A. C. Chandra-Shekara
  • B. M. Prasanna
  • S. R. Bhat
  • B. B. Singh
Article

DOI: 10.1007/BF03321924

Cite this article as:
Chandra-Shekara, A.C., Prasanna, B.M., Bhat, S.R. et al. J. Plant Biochem. Biotechnol. (2007) 16: 23. doi:10.1007/BF03321924

Abstract

Pearl millet [Pennisetum glaucum (L) R Br] is one of the widely grown cereal crops in the arid and semi-arid regions of Africa and India. We undertook a study to ascertain the genetic diversity in 21 elite inbreds (parental lines of 13 pearl millet hybrids in India) using 20 Random Amplified Polymorphic DNA (RAPD) and 21 Simple Sequence Repeat (SSR) markers. Based on Polymorphism Information Content (PIC) and unique banding profiles, 6 RAPD primers OPD12, OPA16, OPB6, OPA19, OPB5 and OPB1, and 3 SSR markers Xpsmp2208, Xpsmp2223 and Xpsmp2220, were found to be highly discriminative. The PIC values ranged from 0.28 to 0.48 for the RAPD and from 0.24 to 0.60 for the SSR markers. Cluster analysis and principal component analysis of the combined dataset of RAPD and SSR markers indicated moderate genetic divergence among the elite pearl millet germplasm, besides unraveling the genetic relationships among the male sterile lines and the restorers.

Key words

DNA fingerprintinggenetic diversityPennisetum glaucumRAPDSSR

Abbreviations

PCA

Principal Component Analysis

PCR

Polymerase Chain Reaction

GS

Genetic Similarity

PIC

Polymorphism Information Content

RAPD

Random Amplified Polymorphic DNA

SSR

Simple Sequence Repeat

UPGMA

Unweighted Pair Group Method using Arithmetic Averages

Copyright information

© Springer 2007

Authors and Affiliations

  • A. C. Chandra-Shekara
    • 1
  • B. M. Prasanna
    • 1
  • S. R. Bhat
    • 2
  • B. B. Singh
    • 1
  1. 1.Division of GeneticsIndian Agricultural Research InstituteNew DelhiIndia
  2. 2.National Research Center on Plant BiotechnologyIndian Agricultural Research InstituteNew DelhiIndia
  3. 3.Department of Plant PathologyUniversity of KentuckyLexingtonUSA