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Agrobacterium-mediated transformation of yellow lupin to generate callus tissue producing HBV surface antigen in a long-term culture

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Abstract

The idea of an oral vaccine administered as a portion of plant tissue requires a high level of antigen production. An improved protocol for the induction of transgenic yellow lupin calli or tumours, reaching 44% of transformation rate, is presented here. It has been developed by using thenptII marker gene and theuidA reporter gene as well as variousAgrobacterium strains and plant explants. This method of seedling and hypocotyl transformation was applied to raise calli or tumours producing a small surface antigen of Hepatitis B Virus (S-HBsAg). Lupin tissue lines were long-term cultured on selection media maintaining the growth rate and high expression level of the native form of S-HBs, up to 6 μg per g of fresh tissue.

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Authors and Affiliations

  1. Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland

    Tomasz Pniewski & Józef Kapusta

  2. Institute of Biotechnology and Antibiotics, Warszawa, Poland

    Józef Kapusta & Andrzej Płucienniczak

Authors
  1. Tomasz Pniewski
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  2. Józef Kapusta
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  3. Andrzej Płucienniczak
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Correspondence to Tomasz Pniewski.

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These authors contribute to this work equally

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Pniewski, T., Kapusta, J. & Płucienniczak, A. Agrobacterium-mediated transformation of yellow lupin to generate callus tissue producing HBV surface antigen in a long-term culture. J Appl Genet 47, 309–318 (2006). https://doi.org/10.1007/BF03194640

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  • DOI: https://doi.org/10.1007/BF03194640

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