Allele Frequencies and Molecular Genotyping of the ABO Blood Group System in a Kuwaiti Population

  • Suzanne Al-Bustan
  • Mokhtar El-Zawahri
  • Daleil Al-Azmi
  • Abdul-Aziz Al-Bashir
Progress in Hematology

DOI: 10.1007/BF02982019

Cite this article as:
Al-Bustan, S., El-Zawahri, M., Al-Azmi, D. et al. Int J Hematol (2002) 75: 147. doi:10.1007/BF02982019

Abstract

The phenotypic distributions of observed numbers of ABO blood groups in a Kuwaiti sample population of 18,558 subjects are 4962 (26.7%) with A, 4462 (24.1%) with B, 858 (4.6%) with AB, and 8276 (44.6%) with O. The calculated gene frequencies are 0.6678 for ABO*O, 0.1768 for ABO*A, and 0.1554 for ABO*B. Molecular genotyping of the ABO blood group system in a Kuwaiti sample population was determined using polymerase chain reaction—restriction fragment length polymorphism (PCR-RFLP) analysis. The positions of nucleotides 258 and 700 of cDNA from A transferase were amplified by PCR. The amplified DNA was subjected to RFLP analysis to distinguish A, B, and O alleles. Blood samples of known ABO phenotype from 101 healthy unrelated Kuwaiti individuals (A, 29; B, 23;AB, 14; O, 35) were used.Two DNA fragments of the ABO locus were designed to be amplified by 2 pairs of primers. To identify the 258th nucleotide, a 199- or 200-bp DNA fragment was amplified by PCR and digested withKpnI. For the 700th nucleotide, a 128-bp DNA fragment was amplified by PCR and digested withAluI. By analyzing the electrophoresis patterns,ABO genotypes were conclusively determined by examining the DNA fragments. The ABO genotypes of the known 101 samples were as follows:AA, 4.30%;AO, 24.41%;BB, 4.16%;BO, 24.2%;AB, 8.46%; andOO, 34.65%. These results were confirmed statistically using the calculated frequencies ofIA,IB, andIO alleles.

Key words

Allele frequencies Molecular ABO Kuwait 

Copyright information

© The Japanese Society of Hematology 2002

Authors and Affiliations

  • Suzanne Al-Bustan
    • 1
  • Mokhtar El-Zawahri
    • 1
  • Daleil Al-Azmi
    • 1
  • Abdul-Aziz Al-Bashir
    • 2
  1. 1.Department of Biological SciencesKuwait UniversitySafat
  2. 2.Central Blood Bank of KuwaitSafatKuwait
  3. 3.Department of Biological Sciences, Faculty of ScienceKuwait UniversitySafatKuwait

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