Articles Drug Devopment

Archives of Pharmacal Research

, Volume 29, Issue 7, pp 566-569

Influence of ginsenoside Rb1 on brain neurosteroid during acute immobilization stress

  • Sang Hee LeeAffiliated withBioanalysis & Biotransformation Research Center, Korea Institute of Science & TechnologyGraduate School of East-West Medical Science, Kyung Hee University
  • , Byung Hwa JungAffiliated withBioanalysis & Biotransformation Research Center, Korea Institute of Science & Technology
  • , Sang Yoon ChoiAffiliated withKorea Food Research Institute
  • , Sun Yeou KimAffiliated withGraduate School of East-West Medical Science, Kyung Hee University
  • , Eunjoo H LeeAffiliated withGraduate School of East-West Medical Science, Kyung Hee University
  • , Bong Chul ChungAffiliated withBioanalysis & Biotransformation Research Center, Korea Institute of Science & Technology Email author 

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Abstract

This study examined whether or not acute stress is linked to increases in the neurosteroid levels, which is a well-known neurotransmitters associated with stress stimuli. The ginsenoside, Rb1, was tested in order to better understand its potential effects on altering the neurosteroid levels and ultimately attenuating stress. The optimal stressed condition was checked by measuring the 5a-dihydroprogesterone (DHP) and allopregnanolone (THP) levels in the brain after immobilization stress at various times. Based on this result, an acute stress model was set up to give 30 min of immobilization stress. The DHP and THP brain levels of the stressed mice were then investigated after administering Rb1 orally (10 mg/kg). These results were compared with the neurosteroid level in the stressed mice not given Rb1. Saline was administered orally to the nonstressed mice to check the placebo effect. Acute immobilization stress induced an increase in the THP and DHP concentration in the frontal cortex and cerebellum. When Rb1 was administered orally prior to immobilization stress, the THP level in the frontal cortex and cerebellum was significantly lower than that in the stressed animals not given Rb1. On the other hand, the DHP level was lower in the cerebellum only. This suggests that the metabolism of the brain neurosteroids in linked to psychological stress, and Rb1 attenuates the stress-induced increase in neurosteroids.

Key words

Immobilization stress Neurosteroid Frontal cortex Cerebellum Ginsenoside Rb1 GC-MS