Archives of Pharmacal Research

, Volume 29, Issue 7, pp 566–569

Influence of ginsenoside Rb1 on brain neurosteroid during acute immobilization stress

Authors

  • Sang Hee Lee
    • Bioanalysis & Biotransformation Research CenterKorea Institute of Science & Technology
    • Graduate School of East-West Medical ScienceKyung Hee University
  • Byung Hwa Jung
    • Bioanalysis & Biotransformation Research CenterKorea Institute of Science & Technology
  • Sang Yoon Choi
    • Korea Food Research Institute
  • Sun Yeou Kim
    • Graduate School of East-West Medical ScienceKyung Hee University
  • Eunjoo H Lee
    • Graduate School of East-West Medical ScienceKyung Hee University
    • Bioanalysis & Biotransformation Research CenterKorea Institute of Science & Technology
Articles Drug Devopment

DOI: 10.1007/BF02969266

Cite this article as:
Lee, S.H., Jung, B.H., Choi, S.Y. et al. Arch Pharm Res (2006) 29: 566. doi:10.1007/BF02969266

Abstract

This study examined whether or not acute stress is linked to increases in the neurosteroid levels, which is a well-known neurotransmitters associated with stress stimuli. The ginsenoside, Rb1, was tested in order to better understand its potential effects on altering the neurosteroid levels and ultimately attenuating stress. The optimal stressed condition was checked by measuring the 5a-dihydroprogesterone (DHP) and allopregnanolone (THP) levels in the brain after immobilization stress at various times. Based on this result, an acute stress model was set up to give 30 min of immobilization stress. The DHP and THP brain levels of the stressed mice were then investigated after administering Rb1 orally (10 mg/kg). These results were compared with the neurosteroid level in the stressed mice not given Rb1. Saline was administered orally to the nonstressed mice to check the placebo effect. Acute immobilization stress induced an increase in the THP and DHP concentration in the frontal cortex and cerebellum. When Rb1 was administered orally prior to immobilization stress, the THP level in the frontal cortex and cerebellum was significantly lower than that in the stressed animals not given Rb1. On the other hand, the DHP level was lower in the cerebellum only. This suggests that the metabolism of the brain neurosteroids in linked to psychological stress, and Rb1 attenuates the stress-induced increase in neurosteroids.

Key words

Immobilization stressNeurosteroidFrontal cortexCerebellumGinsenoside Rb1GC-MS

Copyright information

© The Pharmaceutical Society of Korea 2006