Abstract
Apfl ldhA double mutantEscherichia coli strain NZN111 was used to produce succinic acid by overexpressing theE. coli malic enzyme gene (sfcA). This strain, however, produced a large amount of malic acid as well as succinic acid. After the analyses of the metabolic pathways, thefumB gene encoding the anaerobic fumarase ofE. coli was co-amplified to solve the problem of malic acid accumulation. A plasmid, pTrcMLFu, was constructed, which contains an artificial operon (sfcA-fumB) under the control of the inducibletrc promoter. From the batch culture of recombinantE. coli NZN111 harboring pTrcMLFu, 7 g/L of succinic acid was produced from 20 g/L of glucose, with no accumulation of malic acid. From the metabolic flux analysis the strain was found under reducing power limiting conditions by severe reorientation of metabolic fluxes.
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Hong, S.H., Lee, S.Y. Enhanced production of succinic acid by metabolically engineeredEscherichia coli with amplified activities of malic enzyme and fumarase. Biotechnol. Bioprocess Eng. 9, 252–255 (2004). https://doi.org/10.1007/BF02942339
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DOI: https://doi.org/10.1007/BF02942339