Biotechnology and Bioprocess Engineering

, Volume 9, Issue 2, pp 137–142

Microcontact printing of biotin for selective immobilization of streptavidin-fused proteins and SPR analysis

  • Jong Pil Park
  • Seok Jae Lee
  • Tae Jung Park
  • Kyung-Bok Lee
  • Insung S. Choi
  • Sang Yup Lee
  • Min-Gon Kim
  • Bong Hyun Chung
Article

DOI: 10.1007/BF02932997

Cite this article as:
Park, J.P., Lee, S.J., Park, T.J. et al. Biotechnol Bioproc E (2004) 9: 137. doi:10.1007/BF02932997
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Abstract

In this study, a simple procedure is described for patterning biotin on a glass substrate and then selectively immobilizing proteins of interest onto the biotin-patterned surface. Microcontact printing (μCP) was used to generate the micropattern of biotin and to demonstrate the selective immobilization of proteins by using enhanced green fluorescent protein (EGFP) as a model protein, of which the C-terminus was fused to a core streptavidin (cSA) gene ofStreptomyces avidinii. Confocal fluorescence microscopy was used to visualize the pattern of the immobilized protein (EGFP-cSA), and surface plasmon resonance was used to characterize biological activity of the immobilized EGFP-cSA. The results suggest that this strategy, which consists of a combination of μμCP and cSA-fused proteins, is an effective way for fabricating biologically active substrates that are suitable for a wide variety of applications, one such being the use in protein-protein assays.

Keywords

microcontact printing (μCP) pattern generation protein-protein assay surface plasmon resonance 

Copyright information

© The Korean Society for Biotechnology and Bioengineering 2004

Authors and Affiliations

  • Jong Pil Park
    • 1
  • Seok Jae Lee
    • 1
  • Tae Jung Park
    • 1
  • Kyung-Bok Lee
    • 3
  • Insung S. Choi
    • 3
  • Sang Yup Lee
    • 1
    • 2
  • Min-Gon Kim
    • 4
  • Bong Hyun Chung
    • 4
  1. 1.Department of Chemical and Biomolecular Engineering, Bioprocess Engineering Research Center, Center for Ultramicrochemical Process SystemsKorea Advanced Institute of Science and TechnologyDaejeonKorea
  2. 2.Department of Chemical and Biomolecular Engineering, Bioprocess Engineering Research Center, Department of BioSystems, Bioinformatics Research CenterKorea Advanced Institute of Science and TechnologyDaejeonKorea
  3. 3.Department of ChemistryKorea Advanced Institute of Science and TechnologyDaejeonKorea
  4. 4.BioNanotechnology Research CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonKorea

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