Article

Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 24, Issue 6, pp 630-632

Immunohistochemical analysis of TNF-α and HSP-60 in women with tubal factor infertility associated withChlamydia trachomatis

  • Zhao HaizhenAffiliated withDepartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
  • , Li HongfaAffiliated withDepartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

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Summary

To explore the roles of tumor necrosis factor-α (TNF-α) and heat shock protein 60 (HSP-60) in women with tubal factor infertility (TFI) associated withChlamydia trachomatis, and to determine the mechanisms of fallopian adhesions inChlamydia trachomatis (CT) infections, the expressions of TNF-α and HSP-60 were quantitatively determined in 60 cases of TFI and 30 controls by immunohistochemical technique. The patients with TFI were further divided into group A and group B according to the CT-DNA of cervical specimens of PCR. The quantitative analysis was conducted by employing computerized image analysis system. It is found that the expressions of TNF-α and HSP-60 were much higher in TFI patients than those of controls. Among CT-HSP responders, a stronger expression was correlated with more severe salpingeal pathology. It is concluded that TNF-α and HSP-60 play very important roles in fallopian tube adhesion and occlusion in TFI due to CT infection.

Key words

TFI HSP-60 TNF-α Chlamydia trachomatis immunohistochemistry image analysis