Summary
A diethylnitrosamine-induced hepatocellular carcinoma cell suspension (line-10), injected intraperitoneally in Sewall Wright strain-2 guinea pigs, causes ascites with implantation of malignant cells on the peritoneal surface. At these sites, swelling of the mesothelial cells and simultaneous proliferation of underlying fibroblasts and capillaries are seen. When there are about four layers of malignant cells and the mesothelial lining is disrupted, papillary projections of fibroblasts with capillaries, covered by malignant cells develop. These begin to behave as a “tissue”. In these areas basement membrane destruction and lymphatic and blood vessel infiltration are demonstrable. These developments have been investigated by light microscopy, histochemistry, transmission- and scanning electron microscopy.
Similar content being viewed by others
References
Ashley DJB (1978) Evans’ histological appearances of tumours. Churchill Livingstone, Edinburgh
Bales CE, Durfee GR (1979) Cytologic techniques. In: Koss LG (ed) Diagnostic cytology and its histopathologic bases. Toronto JB Lippincott Company Philadelphia, pp 1187–1266
Birbeck MSC, Wheatley DN (1965) An electron microscopic study of the invasion of ascites tumor cells into the abdominal wall. Cancer Res 25:490–497
Buck RC (1973) Walker 256 tumor implantation in normal and injured peritoneum studied by electron microscopy, scanning electron microscopy, and autoradiography. Cancer Res 33:3181–3188
Carr J, Carr I, Dreher B, Betts K (1980) Lymphatic metastasis: invasion of lymphatic vessels and efflux of tumour cells in the afferent popliteal lymph as seen in the Walker rat carcinoma. J Pathol 132:287–305
Foyle A, Al-Jabi M, Elliot McCaughey WT (1981) Papillary peritoneal tumors in women. Am J Surg Pathol 5:241–249
Huth F, Pfitzer P (1973) Electron microscopy in cytological diagnosis of effusions. Verh Dtsch Ges Pathol 57:275–279
Jap P, Willart E, van de Molengraft F, Vooijs G (1981) Changes of surface configurations and behaviour of stimulated mesothelial cells. Beitr Elektronenmikr Direktabb Oberfl 14:499–502
Lojda Z, Gossrau R, Schiebler TH (1976) Enzymhistochemische Methoden. Springer, Berlin Heidelberg New York
Lunscken CH, Sträuli P (1975) Penetration of an ascitic reticulum cell sarcoma of the golden hamster into the body wall and through the diaphragm. Virchows Arch [Cell Pathol] 17:247–259
van de Molengraft F, Jap P, Willart E, Wirtz P, Vooijs G (1981) SEM and TEM of the invasive process in induced carcinosis peritonei (guinea pig). Beitr Elektronenmikr Direktabb Oberfl 14:503–506
Mungyer G, Jap P, van de Molengraft F (1981) Growing squamous bovine lens epithelial cells “in vitro” as a “target” for tumor cells of hepatocellular origin (guinea pig). Abstracts of the 2nd Metastasis Conference, European Organization for Research in Treatment of Cancer Amsterdam
Rapp HJ, Churchill WH, Kronman BS, Rolley RT, Hammond WG, Borsos T (1968) Antigenicity of a new diethylnitrosamine-induced transplantable guinea pig hepatoma: pathology and formation of ascites variant. J Natl Cancer Inst 41:1–11
Smith WE, Hubert DD, Holiat SM, Sobel HJ, Davis S (1981) An experimental model for treatment of mesothelioma. Cancer 47:658–663
Sträuli P, Haemmerli G, Tschenett C, Krstic RV (1981) Different modes of mesenteric infiltration displayed by two rat leukemias. Virchows Arch [Cell Pathol] 35:93–108
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
van de Molengraft, F., Jap, P., Wirtz, P. et al. Observations on induced carcinosis peritonei of the guinea pig. Virchows Archiv B Cell Pathol 39, 87–99 (1982). https://doi.org/10.1007/BF02892839
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02892839