Article

Virchows Archiv B

, Volume 39, Issue 1, pp 87-99

First online:

Observations on induced carcinosis peritonei of the guinea pig

  • Fred van de MolengraftAffiliated withSection of Cytopathology, Institute for Pathologic Anatomy, University of Nijmegen
  • , Paul JapAffiliated withDepartment of Cytology and Histology, University of Nijmegen
  • , Peter WirtzAffiliated withDepartment of Cytology and Histology, University of Nijmegen
  • , Peter VooijsAffiliated withSection of Cytopathology, Institute for Pathologic Anatomy, University of Nijmegen

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Summary

A diethylnitrosamine-induced hepatocellular carcinoma cell suspension (line-10), injected intraperitoneally in Sewall Wright strain-2 guinea pigs, causes ascites with implantation of malignant cells on the peritoneal surface. At these sites, swelling of the mesothelial cells and simultaneous proliferation of underlying fibroblasts and capillaries are seen. When there are about four layers of malignant cells and the mesothelial lining is disrupted, papillary projections of fibroblasts with capillaries, covered by malignant cells develop. These begin to behave as a “tissue”. In these areas basement membrane destruction and lymphatic and blood vessel infiltration are demonstrable. These developments have been investigated by light microscopy, histochemistry, transmission- and scanning electron microscopy.

Key words

Ascites Hepatocarcinoma Neoplasm metastasis Neoplasms experimental