Virchows Archiv B

, Volume 45, Issue 1, pp 385–396

Immunohistochemical study on the distribution of α and β subunits of S-100 protein in human neoplasm and normal tissues

  • Kiyoshi Takahashi
  • Toshiaki Isobe
  • Yuji Ohtsuki
  • Tadaatsu Akagi
  • Hiroshi Sonobe
  • Tsuneo Okuyama
Article

DOI: 10.1007/BF02889881

Cite this article as:
Takahashi, K., Isobe, T., Ohtsuki, Y. et al. Virchows Archiv B Cell Pathol (1984) 45: 385. doi:10.1007/BF02889881

Summary

The immunohistochemical distribution and localization of the α and β subunits of S-100 protein in human neoplasms and normal tissues were studied by the PAP method using monospecific rabbit antibodies against each subunit, β subunit immunoreactivity was detected in all S-100-positive cells and tumors reported previously. In contrast a subunit immunoreactivity was absent from Schwann cells, schwannomas, neurofibromas, granular cell myoblastomas, pituicytes of the neurohypophysis, Langerhans cells, interdigitating reticulum cells, and histiocytosis X cells. Interestingly, only the α subunit was detected in neurons of both central and peripheral nervous system, and in lymph node macrophages. Human S-100-positive cells are divided into three groups; the first is composed of cells containing only the β subunit (probably S-100 b; ββ), the second consists of cells containing both the a and β subunits, and the third is composed of cells containing only the α subunit (probably S-100ao; αα). The ontogentic relationships between S-100-positive cells and tumors are discussed in the light of these findings.

Key words

S-100 protein, a and β subunitsImmunohistochemistryHuman neoplasmHuman normal tissues

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Kiyoshi Takahashi
    • 1
  • Toshiaki Isobe
    • 3
  • Yuji Ohtsuki
    • 1
  • Tadaatsu Akagi
    • 1
  • Hiroshi Sonobe
    • 2
  • Tsuneo Okuyama
    • 3
  1. 1.Department of PathologyKochi Medical SchoolKochi
  2. 2.Department of Central LaboratoryKochi Medical SchoolJapan
  3. 3.Department of Chemistry, Faculty of ScienceTokyo Metropolitan UniversityTokyoJapan