Article

Virchows Archiv B

, Volume 45, Issue 1, pp 385-396

Immunohistochemical study on the distribution of α and β subunits of S-100 protein in human neoplasm and normal tissues

  • Kiyoshi TakahashiAffiliated withDepartment of Pathology, Kochi Medical School
  • , Toshiaki IsobeAffiliated withDepartment of Chemistry, Faculty of Science, Tokyo Metropolitan University
  • , Yuji OhtsukiAffiliated withDepartment of Pathology, Kochi Medical School
  • , Tadaatsu AkagiAffiliated withDepartment of Pathology, Kochi Medical School
  • , Hiroshi SonobeAffiliated withDepartment of Central Laboratory, Kochi Medical School
  • , Tsuneo OkuyamaAffiliated withDepartment of Chemistry, Faculty of Science, Tokyo Metropolitan University

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Summary

The immunohistochemical distribution and localization of the α and β subunits of S-100 protein in human neoplasms and normal tissues were studied by the PAP method using monospecific rabbit antibodies against each subunit, β subunit immunoreactivity was detected in all S-100-positive cells and tumors reported previously. In contrast a subunit immunoreactivity was absent from Schwann cells, schwannomas, neurofibromas, granular cell myoblastomas, pituicytes of the neurohypophysis, Langerhans cells, interdigitating reticulum cells, and histiocytosis X cells. Interestingly, only the α subunit was detected in neurons of both central and peripheral nervous system, and in lymph node macrophages. Human S-100-positive cells are divided into three groups; the first is composed of cells containing only the β subunit (probably S-100 b; ββ), the second consists of cells containing both the a and β subunits, and the third is composed of cells containing only the α subunit (probably S-100ao; αα). The ontogentic relationships between S-100-positive cells and tumors are discussed in the light of these findings.

Key words

S-100 protein, a and β subunits Immunohistochemistry Human neoplasm Human normal tissues