Abstract
The investigation of the substrate specificity of the anionic peroxidase isoenzymes, isolated from the zone of differentiation of the primary roots ofZea mays, for some representatives of phenolic compounds and aromatic amines, as hydrogen donors, is reported. The investigation was carried out electrophoretically with peroxidase isoenzymes partially purified by a combination of gel filtration by Sephadex G-25 and Sephadex G-100. A difference in the substrate specificity of the individual isoenzymes is observed. It was established that the anionic peroxidase isoenzymes showed a similarity in total number and relative activity on staining with bivalent phenols and difference on staining with trivalent phenols, as hydrogen donors. A greater number of isoenzymes was stained with benzidine ando-dianisidine and a lesser number witho- andp-phenylendiamine. The substrate specificity of the peroxidase isoenzymes was compared for guaiacol and benzidine. The substrate specificity of peroxidase soenzymes was discussed as regards their diverse role in the plant metabolism.
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Dencheva, A. V., Klisurska, D. J.: [Extraction and partial purification of maize peroxidase in the presence of phenols]. In Bulg. - Fiziol. Rast. (Sofia)1: 14–21, 1979.
Evans, J. J., Alldridge, N. A.: The distribution of peroxidases in extreme dwarf and normal tomato (Lycopersicon esculentum Mill.). - Phytochemistry4: 499–503, 1965.
Farkas, G. L., Stahmann, M. A.: On the nature of changes in peroxidase isoenzymes in bean leaves infected by Southern Bean Mosaic Virus. - Phytopathology56: 669–677, 1966.
Gordon, R. W., Henderson, J. H. M.: Isoperoxidases of IAA — oxidase in oat coleoptiles. - Can. J. Bot.51: 2047–2052, 1973.
Kay, E., Shannon, L. M., Lew, J. Y.: Peroxidase isoenzymes from horseradish roots. II. Catalytic properties. - J. biol. Chem.242: 2470–2473, 1967.
Macko, V., Novacký, A.: Contribution to the study of plant peroxidase isoenzymes by means of disc electrophoresis on acrylamide gel. - Biológia (Bratislava)21: 128–133, 1966.
Marklund, S., Ohlsson, P.-I, Opara, A., Paul, K.-G.: The substrate profiles of the acidic and slightly basic horseradish peroxidases. - Biochem. biophys. Acta350: 304–313, 1974.
Maurer, H. R.: Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis. - De Gruyter, Berlin 1971.
Pruidze, G. N., Grigorashvili, G. Z., Chachua, L. Sh., Tokhadze, M. V.: [Purification and properties of peroxidase from tea leaves]. In Russ. - Biokhimiya41: 1819–1828, 1976.
Rokitskiï, F. P.: [Biological Statistics]. In Russ. - Vysshaya Shkola, Minsk 1967.
Rubin, B. A., Lushinskaya, E. N.: [Dynamics of the components of peroxidase in the leaves of corn plant affected by brandUstilago zeae Unger]. In Russ. -Biokhimiya34: 677 to 679, 1969.
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Klisurska, D., Dencheva, A. Substrate specificity of peroxidase isoenzymes for hydrogen donors. Biol Plant 22, 404–409 (1980). https://doi.org/10.1007/BF02880476
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DOI: https://doi.org/10.1007/BF02880476