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Marker-assisted genetic analysis of non-acclimated freezing tolerance and cold acclimation capacity in a backcrossSolarium population

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Abstract

Random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers were used to construct a partial genetic linkage map in a potato backcross population. The population, derived from two diploid wildSolatium species (frost tolerant, able to cold acclimateS. commersonii; frost sensitive, unable to cold acclimateS. cardiophyllum), was used to map quantitative trait loci (QTL) of non-acclimated relative freezing tolerance (NARFT) and cold acclimation capacity (CAC). Precise assessment of these traits allowed distinction of small but significant differences among 35 backcross genotypes. NARFT and CAC were not correlated in the segregating population, suggesting independent genetic control for these two major components of freezing tolerance. The linkage map spanned 479.4 cM and included 77 RAPD markers and two SSR markers, with 38 RAPD and 10 SSR unassigned markers. Two QTLs for NARFT were detected in two different linkage groups, accounting for 44.0% of the phenotypic variation for this trait. Two QTLs for CAC were detected, accounting for 24.9% of the phenotypic variation for this trait. QTLs for NARFT and CAC were detected at separate genomic regions, in support of the independent genetic control of these two traits. QTLs for NARFT and CAC were detected in a linkage group identified as part of chromosome V, suggesting that such chromosome constitutes a prime candidate for fine-mapping. Due to the relatively small progeny size evaluated in this study, additional QTLs for NARFT and CAC could have been involved but not identified. Therefore, the conclusions derived from this study should be considered preliminary.

Resumen

Marcadores de DNA polimórfico amplificado al azar (RAPD) y de secuencias simples repetidas (SSR) fueron utilizadas para construir un mapa parcial de ligamiento genético en una población de retro-cruza de papa. Esta población fue derivada de dos especies silvestres diploides,S. commersonii (resistente a heladas, capaz de acclimatarse a bajas temperaturas) yS. cardiophyllum (sensible a heladas, incapaz de aclimatarse a bajas temperaturas), y se utilizó para mapear loci de carácteres cuantitativos (QTL) para la tolerancia relativa a heladas sin aclimatación a bajas temperaturas (NARFT) y la capacidad de aclimatarse a bajas temperaturas (CAC). La determinacién precisa de estos dos caracteres permitió encontrar diferencias pequeñas pero significativas entre los 35 genotipos de la retro-cruza. La correlación entre NARFT y CAC no fue significativa en la población segregante, sugieriendo que existe un control genético independiente para cada uno de estos dos componentes mayores de la tolerancia a heladas. El tamaño del mapa fue de 479.4cM e incluyó 77 marcadores RAPD y dos marcadores SSR; con 38 marcadores RAPD y 10 marcadores SSR sin asignar. Para NARFT, dos QTLs fueron detectados en dos diferentes grupos de ligamiento representando 44.0% de la variación fenotípica observada en este carácter. Para CAC, dos QTLs fueron detectados, los cuales representaron 24.9% de la variación fenotípica observada en este carácter. Los QTLs para NARFT y CAC fueron detectados en diferentes regiones genomicas, confirmando el control genético independiente de estos dos caracteres. Los QTLs para NARFT y CAC fueron detectados en un grupo de ligamiento identificado como parte del cromosoma V, sugiriendo que dicho cromosoma podria ser un candidato principal para mapeo fino. Debido a que el tamano de la población evaluada en este estudio fue relativamente pequeño, QTLs adicionales para NARFT y CAC podrían existir. En consecuencia, las conclusiones derivadas de este estudio deben ser consideradas como preliminares.

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Abbreviations

ACCRFT:

acclimated relative freezing tolerance

BC:

backcross

bp:

base pair

CAC:

cold acclimation capacity

GA:

gibberellic acid

LG:

linkage group

LOD:

logarithm of the odds

NARFT:

nonacclimated relative freezing tolerance

PAR:

photosynthetically active radiation

r:

recombinant fraction

RFT:

relative freezing tolerance

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Authors and Affiliations

  1. Department of Horticulture, University of Wisconsin, 1575 Linden Drive, 53706, Madison, WI

    Sandra E. Vega, Alfonso H. del Rio & Jiwan P. Palta

  2. Department of Plant Pathology, University of Wisconsin, 1630 Linden Drive, 53706, Madison, WI

    Geunhwa Jung

  3. USDA/Agricultural Research Service, Inter-Regional Potato Introduction Station, 4312 Hwy 42, 54235, Sturgeon Bay, WI

    John B. Bamberg

Authors
  1. Sandra E. Vega
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  2. Alfonso H. del Rio
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  3. Geunhwa Jung
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  4. John B. Bamberg
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  5. Jiwan P. Palta
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Correspondence to Jiwan P. Palta.

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Vega, S.E., del Rio, A.H., Jung, G. et al. Marker-assisted genetic analysis of non-acclimated freezing tolerance and cold acclimation capacity in a backcrossSolarium population. Am. J. Pot Res 80, 359–369 (2003). https://doi.org/10.1007/BF02854247

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