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Regeneration ofSolanum tuberosum cv. Katahdin from leaf expiantsin vitro

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Abstract

A previously published medium has been found to be effective for plant regeneration from leaf expiants ofSolanum tuberosum cv. Katahdin. A number of combinations of thidiazuron and naphthaleneacetic acid, along with a medium previously published for shoot regeneration from protoplast-derived calli ofSolanum phureja, which contained zeatin, indoleacetic acid and gibberellic acid (ZIG), were tested for callus induction, and all calli were transferred to ZIG for shoot induction. We conclude that leaf expiants cultured on ZIG medium at all stages of culture were the most effective at shoot production. A mean of over six shoots were produced per expiant after four months in culture.

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Literature Cited

  • Cheng, J. and R.E. Veilleux. 1991. Genetic analysis of protoplast culturability inSolanum phureja. Plant Science 75: 257–265.

    Article  Google Scholar 

  • Haberlach, G.T., B.A. Cohen, N.A. Reichert, M.A. Baer, L.E. Towill, and J.P. Helgeson. 1985. Isolation, culture, and regeneration of protoplasts from potato and several relatedSolanum species. Plant Science 39: 67–74.

    Article  Google Scholar 

  • Lucier, G., A. Budge, C. Plummer, and C. Spurgeon. 1990. U.S. Potato Statistics, 1949–1989. United States Department of Agriculture Statistical Bulletin 829:98 (table 67).

  • Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15: 473–497.

    Article  CAS  Google Scholar 

  • Park, Y.D., D.H. Ronis, A.A. Boc, and Z.M. Cheng. 1995. Plant regeneration from leaf tissues of four North Dakota genotypes of potato (Solanum tuberosum L.). Am Potato J 72: 329–338.

    Article  CAS  Google Scholar 

  • Seabrook, J.E., S. Coleman, and D. Levy. 1993. Effect of photoperiod onin vitro tuberization of potato (Solanum tuberosum L.). Plant Cell Tissue and Organ Culture 34: 43–51.

    Article  Google Scholar 

  • Walker, J.C. Plant Pathology, McGraw-Hill, Inc., New York, p. 150, 1957, 2nd ed.

    Google Scholar 

  • Wheeler, V.A., N.E. Evans, D. Fougler, K.J. Webb, A. Karp, J. Franklin, and S.W.J. Bright. 1985. Shoot formation from expiant cultures of fourteen potato cultivars and studies of the cytology and morphology of regenerated plants. Annals of Botany 55: 321–329.

    Google Scholar 

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Author notes

  1. J. A. Cearley

    Present address: Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 35294, Birmingham, AL

Authors and Affiliations

  1. Department of Biological Sciences, Southern Illinois University at Edwardsville, 62026-1651, Edwardsville, IL

    M. G. Bolyard

Authors
  1. J. A. Cearley
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  2. M. G. Bolyard
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Cearley, J.A., Bolyard, M.G. Regeneration ofSolanum tuberosum cv. Katahdin from leaf expiantsin vitro . American Potato Journal 74, 125–129 (1997). https://doi.org/10.1007/BF02851558

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  • DOI: https://doi.org/10.1007/BF02851558

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