Skip to main content
Log in

Isolation of RNA from blackcurrant (Ribes nigrum L.) fruit

  • Research
  • Published:
Molecular Biotechnology Aims and scope Submit manuscript

Abstract

Extraction of high-quality RNA from blackcurrant fruit has hitherto proved difficult, probably owing to high levels of phenolic and polysaccharide components in the berries. The procedure described here is a modification of one described for grape berries, and yields RNA suitable for in vitro translations, RNA blot analysis, and cDNA library construction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Hobson, G. and Grierson, D. (1993) Tomato, inBiochemistry of Fruit Ripening (Seymour, G. B., Taylor, J. E., and Tucker, G. A., eds.), Chapman and Hall, Cambridge, UK, pp. 405–422.

    Google Scholar 

  2. Given, N. K. (1993) Kiwifruit, inBiochemistry of Fruit Ripening (Seymour, G. B., Taylor, J. E., and Tucker, G. A., eds.), Chapman and Hall, Cambridge, UK, pp. 235–254.

    Google Scholar 

  3. Seymour, G. B. and Tucker, G. A. (1993) Avocado, in:Biochemistry of Fruit Ripening (Seymour, G. B., Taylor, J. E., and Tucker, G. A., eds.), Chapman and Hall, Cambridge, UK, pp. 53–81.

    Google Scholar 

  4. Smith, C. J. S., Watson, C. F., Morris, P. C., Bird, C. R., Seymour, G. B., Gray, J. E., Arnold, C., Tucker, G. A., Schuch, W., Harding, S., and Grierson, D. (1990) Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes.Plant Mol. Biol. 14, 369–379.

    Article  PubMed  CAS  Google Scholar 

  5. Schuch, W., Kanczler, J., Robertson, D., Hobson, G., Tucker, G., Grierson, D., Bright, S., and Bird, C. (1991) Fruit quality characteristics of transgenic tomato fruit with altered polygalacturonase activity.Hortscience 26, 1517–1520.

    CAS  Google Scholar 

  6. Tesniere, C. and Vayda, M. E. (1991) Method for the isolation of high-quality RNA from grape berry tissues without contaminating tannins or carbohydrates.Plant Mol. Biol. Reptr. 9, 242–251.

    CAS  Google Scholar 

  7. Lay-Yee, M., Della-Penna, D., and Ross, G. S. (1990) Changes in mRNA and protein during ripening in apple fruit (Malus domestica Borkh. cv. Golden Delicious).Plant Physiol. 94, 850–853.

    Article  PubMed  CAS  Google Scholar 

  8. Callahan, A., Morgens, P., and Walton, E. (1989) Isolation andin vitro translation of RNAs from developing peach fruit.Hortscience 24, 356–358.

    CAS  Google Scholar 

  9. Newbury, H. J. and Possingham, J. V. (1977) Factors affecting the extraction of intact ribonucleic acid from plant tissues containing interfering phenolic compounds.Plant Physiol. 60, 543–547.

    PubMed  CAS  Google Scholar 

  10. John, M. E. (1992) An efficient method for isolation of RNA and DNA from plants containing polyphenolics.Nucleic Acids Res. 20, 2381.

    Article  PubMed  CAS  Google Scholar 

  11. Schneiderbauer, A., Sanderman, H., and Ernst, D. (1991) Isolation of functional RNA from plant tissues rich in phenolic compounds.Anal. Biochem. 197, 91–95.

    Article  PubMed  CAS  Google Scholar 

  12. Manning, K. (1991) Isolation of nucleic acids from plants by differential solvent precipitation.Anal. Biochem. 195, 45–50.

    Article  PubMed  CAS  Google Scholar 

  13. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989)Molecular Cloning. A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  14. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227, 680–685.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Mark A. Taylor.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Woodhead, M., Taylor, M.A., Davies, H.V. et al. Isolation of RNA from blackcurrant (Ribes nigrum L.) fruit. Mol Biotechnol 7, 1–4 (1997). https://doi.org/10.1007/BF02821541

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02821541

Index Entries

Navigation