In Vitro

, Volume 12, Issue 10, pp 670–677

The origin and characteristics of a pig kidney cell strain, LLC-PK1


  • R. N. Hull
    • Lilly Research LaboratoriesDivision of Eli Lilly and Company
  • W. R. Cherry
    • Lilly Research LaboratoriesDivision of Eli Lilly and Company
  • G. W. Weaver
    • Lilly Research LaboratoriesDivision of Eli Lilly and Company

DOI: 10.1007/BF02797469

Cite this article as:
Hull, R.N., Cherry, W.R. & Weaver, G.W. In Vitro Cell.Dev.Biol.-Plant (1976) 12: 670. doi:10.1007/BF02797469


A stable epithelial-like pig kidney cell strain has been established. This strain has been carried through more than 300 serial passages, has remained free of microbial and viral contaminants, and has retained a near diploid number of chromosomes. Attempts to produce tumors with these cells in immunosuppressed laboratory animals have been uniformly negative. The cells have grown rapidly in monolayer cultures with a split ratio of 1 to 15 at weekly intervals, but have failed to proliferate in suspension cultures. A subline adapted to growth on serum-free medium 199 has been carried through 145 passages on this medium. Several unusual morphologic features have been observed in these cultures including three-dimensional “domelike” structures. These cells have been found susceptible to some viruses and have been especially useful for viruses of domestic animals. LLC-PK1 cells have produced significant levels of plasminogen activator.

Key words

pig kidney cellsdome-like structureschemically defined mediaplasminogen activatorvirus susceptibility

Copyright information

© Society for In Vitro Biology 1977