Summary
A simple method is described for primary culture and for maintenance of hormone-producing cells from normal human placenta. A consistent yield of cells was obtained and an average survival of 3 to 4 months in culture using 1 mm3 explants from the most vascular area of the placentas. These explants were placed in a variety of culture media in 30 ml flasks and incubated at 37°C in an atmosphere of 5% CO2 and 95% air. The best yields in terms of cell growth were observed with Eagle’s MEM (minimum essential medium) with supplements of horse serum and fetal calf serum or human cord serum. (Ham’s F-10 with supplement of horse serum and fetal calf serum supports growth for the longest period and media containing human cord serum had the best yield of steroids.)
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Roy, P.W., Ryan, G.E. & Bransome, E.D. Primary culture and maintenance of steroid-secreting human placental monolayers. In Vitro Cell.Dev.Biol.-Plant 12, 115–119 (1976). https://doi.org/10.1007/BF02796357
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DOI: https://doi.org/10.1007/BF02796357