Skip to main content
Log in

A modification of the phenol/sulfuric acid assay for total carbohydrates giving more comparable absorbances

  • Original Articles
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The conditions and acid strength of the phenol/sulfuric acid assay were investigated to improve agreement between absorbances obtained from different sugars. It was found that by increasing acid strength and by cooling the tubes in water after a short reaction time, the values obtained for several sugars, including fructose and xylose, agreed, on an equimolar value, with that for glucose.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Du Bois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956),Anal. Chem. 28, 350–356.

    Article  Google Scholar 

  2. Rao, P. and Pattabiraman, T. N. (1989),Anal. Biochem. 181, 18–22.

    Article  CAS  Google Scholar 

  3. Rao, P. and Pattabiraman, T. N. (1990),Anal. Biochem. 189, 178–181.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Taylor, K.A.C.C. A modification of the phenol/sulfuric acid assay for total carbohydrates giving more comparable absorbances. Appl Biochem Biotechnol 53, 207–214 (1995). https://doi.org/10.1007/BF02783496

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02783496

Index Entries

Navigation