Skip to main content
Log in

Detectionin situ of endophyte withinTricholoma matsutake/Pinus densiflora mycorrhizal roots

  • Short Communication
  • Published:
Journal of Forest Research

Abstract

Tricholoma matsutake/Pinus densiflora mycorrhizas andP. densiflora roots were collected from beneath matureT. matsutake fruit-bodies in a Shiro in central Japan and investigated for evidence of endophytic infection.Tricholoma matsutake infection was determined microscopically on cleared, bleached and stained mycorrhizas by the presence of both a thin, discontinuous mantle and highly branched, sparingly septate Hartig net mycelium within the root cortex. Endophytic infection of Matsutake mycorrhizas was characterized by intracortical sclerotia, simple septate mycelium within the cortex and vascular cylinder and intracellular spore masses within the root cortex.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Literature cited

  • Bills, G.F. (1996) Isolation and analysis of endophytic fungal communities from woody plants.In Endophytic fungi in grasses and woody plants. Systematics, ecology and evolution. Redlin, S.C. and Carris, L.M. (eds.), 223pp, APS Press, St. Paul, 31–65.

    Google Scholar 

  • Brundrett, M.C., Piché, Y., and Peterson, R.L. (1984) A new method for observing the morphology of vesicular-arbuscular mycorrhizae. Can. J. Bot. 62: 2128–2134.

    Article  Google Scholar 

  • Fitter, A.H. and Garbaye, J. (1994) Interaction between mycorrhizal fungi and other organisms.In Management of mycorrhizas in agriculture, horticulture and forestry. Robson, A.D., Abbott, L.K., and Malajczuk, N. (eds.), 238pp, Kluwer, Dordrecht, 11–25.

    Google Scholar 

  • Gill, W.M. and Suzuki, K. (2000) The external morphological characterization ofTricholoma matsutake infection of hostPinus densiflora lateral roots. J. For. Res. 5: 99–102.

    Google Scholar 

  • Gill, W.M., Lapeyrie, F., Gomi, T., and Suzuki, K. (1999)Tricholoma matsutake—an assessment ofin situ andin vitro infection by observing cleared and stained whole roots. Mycorrhiza 9: 227–231.

    Article  Google Scholar 

  • Gill, W.M., Guerin-Laguette, A., Lapeyrie, F., and Suzuki, K. (2000) Matsutake—morphological evidence of ectomycorrhiza formation betweenTricholoma matsutake and host roots in a purePinus densiflora forest stand. New Phytol. 147: 381–388.

    Article  Google Scholar 

  • Guerin-Laguette, A., Vaario, L.-M., Gill, W.M., Lapeyrie, F., Matsushita, N., and Suzuki, K. (2000) Rapidin vitro ectomycorrhizal infection onPinus densiflora roots byTricholoma matsutake. Mycoscience 41: 389–393.

    Article  Google Scholar 

  • Iwase, K. (1997) Cultivation of mycorrhizal mushrooms. Food Rev. Int. 13: 431–442.

    Article  Google Scholar 

  • Ogawa, M. (1974) Ecology ofTricholoma matsutake (Ito et Imai) Sing., mycorrhizal fungus, in pine forest.In Mushroom science IX. Proceedings of the Ninth International Scientific Congress on the Cultivation of Edible Fungi. Tokyo, 833pp, 733–744.

  • Ogawa, M. (1975a) Microbial ecology of mycorrhizal fungus,Tricholoma matsutake Ito et Imai (Sing.) in pine forest. I. Fungal colony (‘Shiro’) ofTricholoma matsutake. Bull. Gov. For. Exp. Sta. 272: 79–121.

    Google Scholar 

  • Ogawa, M. (1975b) Microbial ecology of mycorrhizal fungi —Tricholoma matsutake (S. Ito et Imai) Sing. in pine forest. II. Mycorrhiza formed byTricholoma matsutake. Bull. Gov. For. Exp. Sta. 278: 21–49.

    Google Scholar 

  • Ogawa, M. (1977) Microbial ecology of mycorrhizal fungus -Tricholoma matsutake (Ito et Imai) Sing. in pine forest. III. Fungal florae in Shiro soil and on the mycorrhiza. Bull. Gov. For. Exp. Sta. 293: 105–170.

    Google Scholar 

  • Wang, Y., Hall, I.R., and Evans, L.A. (1997) Ectomycorrhizal fungi with edible fruiting bodies. I.Tricholoma matsutake and related fungi. Econ. Bot. 51: 311–327.

    Google Scholar 

  • Yamada, A., Kanekawa, S., and Ohmasa, M. (1999) Ectomycorrhiza formation ofTricholoma matsutake onPinus densiflora. Mycoscience 40: 193–198.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

This research was supported by a grant from the Bio-oriented Technology Research Advancement Institution (BRAIN).

About this article

Cite this article

Gill, W.M., Suzuki, K. Detectionin situ of endophyte withinTricholoma matsutake/Pinus densiflora mycorrhizal roots. J For Res 5, 289–292 (2000). https://doi.org/10.1007/BF02767124

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02767124

Key words

Navigation