Abstract
The fluorescence decay of several organic dye molecules intercalated in egg phosphatidylcholine lipid membrane vesicles is consistent with the existence of two or three prominent lifetime components rather than a single continuous distribution of lifetimes. The major lifetime components are identified with different sites of solubilization in the membrane. The variation of the lifetime of the membrane-bound dye was studied as a function of the sucrose concentration, which varied the viscosity and refractive index of the aqueous solution. The combined effect of viscosity and refractive index on the lifetime of the dye was used to identify the site of solubilization of the dye in the membrane. The study was useful to identify dye molecules on the surface which are exposed to the aqueous phase, for which the fluorescence lifetime increased systematically with sucrose (viscosity effect). More importantly, it was possible in a few cases to identify the dye molecules which are oriented in the membrane phase, and the fluorescence lifetime decreased systematically with sucrose (refractive index effect). Anomalous values of order parameters determined from the refractive index effect are explained in terms of an orientational distribution of the linear dye molecule weighted in favor of mutually orthogonal orientations.
Similar content being viewed by others
References
C. D. Stubbs and B. W. Williams (1992) in J. R. Lakowicz (Ed.),Topics in Fluorescence Spectroscopy, Vol. 3, Plenum Press, New York, Chap. 5.
T. G. Dewey (Ed.) (1991)Biophysical and Biochemical Aspects of Fluorescence Spectroscopy, Plenum Press, New York.
B. R. Lentz (1993)Chem. Phys. Lipids 64, 99–116.
J. C. Smith (1990)Biochim. Biophys. Acta 1016, 1–28.
R. P. Haughland (1996)Handbook of Fluorescent Probes and Research Chemicals, 6th ed., Molecular Probes, Eugene, OR.
N. V. Visser, A. van Hoek, A. J. W. G. Visser, J. Frank, H. J. Apell, and R. J. Clarke (1995)Biochemistry 34, 11777–11784.
R. J. Clarke, A. Zouni, and J. F. Holzworth (1995)Biophys. J. 68, 1406–1415.
N. V. Visser, A. van Hoek, A. J. W. G. Visser, R. J. Clarke, and J. F. Holzworth (1994)Chem. Phys. Lett. 231, 551–560.
D. Toptygin and L. Brand (1993)Biophys. Chem. 48, 205–220.
D. Toptygin and L. Brand (1995)J. Fluoresc. 5, 39–50.
G. Krishnamoorthy (1996)Biochemistry 25, 6666–6671.
N. Periasamy, S. Doraiswamy, B. G. Maiya, and B. Venkataraman (1988)Chem. Phys. 88, 1638–1651.
A. K. Livesey and J. C. Brochon (1987)Biophys. J. 52, 693–706.
J. C. Brochon (1994)Methods Enzymol. 240, 262–311.
J. B. Birks (1970)Photophysics of Aromatic Molecules, John Wiley, New York, p. 88.
H. Ephardt and P. J. Fromherz (1989)J. Phvs. Chem. 93, 7717–7725.
P. F. Aramandia, R. M. Negri, and E. S. Roman (1994)J. Phvs. Chem. 98, 3165–3173.
W. Lukosz (1980)Phys. Rev. B 15, 3030–3038.
R. C. Weast (Ed.) (1975)CRC Handbook of Chemistry and Physics, 55th ed., CRC Press, Cleveland, OH, pp. D194-D236.
U. A. van der Heide, G. van Ginkel, and Y. K. Levine (1996)Chem. Phys. Lett. 253, 118–122.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Krishna, M.M.G., Periasamy, N. Fluorescence of organic dyes in lipid membranes: Site of solubilization and effects of viscosity and refractive index on lifetimes. J Fluoresc 8, 81–91 (1998). https://doi.org/10.1007/BF02758241
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02758241