Anti-tumor effect of CDA-II on a human glioma cell
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To examine the effect of uroacitide (CDA-II ), an extraction product from normal human urine, on proliferation and differentiation of human glioma SWO-38 cells.
The effects of CDA-II on cellular survival and colony formation were determined by MTT and colony-formation assays. The in vivo anti-tumor effect of CDA-II was examined on transplanted SWO-38 cells in nude mice. In addition, the aterations in cell morphology induced by CDA-II were observed by H&E staining.
Treatment of SWO-38 cells with 1–5 mg/ml of CDA-II for 3 days, resulted in 39.49%± 5.27%~65.05%± 5.89% growth inhibition with an IC50of 2.52 mg/ml. Based on the colony-formation assay, 10 days of CDA-II treatment at a level of 0.3~2.1 mg/ml caused 23.45%± 0.62%~96.22%±1.01% inhibition with an IC50 of 1.03 mg/ml. Furthermore, the inhibitory response was dose-dependent. CDA-II at dosage of 500 mg/kg or 2,000 mg/kg per day for 4 weeks significantly suppressed the growth of human glioma SWO-38 cells in nude mice, with inhibition being 47.77% and 79.94%, respectively (P< 0.05, n=10). H&E staining and light microscopy revealed that CDA-II induced differentiation of the SWO-38 cells.
CDA-II has a significant anti-tumor effect on the human glioma SWO-38 cells, and is a potential and natural anti-glioma therapeutic reagent.
- Mizunno, M, Yoshida, J (1996) Tumor necrosis factor-α gene transfer augments anti-fas antibody-mediated apoptosis in human glioma cell. Jpn Cancer Res. 87: pp. 543-547
- Liao, MC (1999) A clever anticancer drug CDA-H. ShiMao publisher, Taipei
- Zhong, XY, Chen, YX, Ye, SF (2000) Establishment of two cell sublines from SWO-38 glioma cells: an immunohisto-chemical and ultrastructural Study. Int J Modern Cancer Therapy 3: pp. 34-37
- Marcantonio, D, Panasci, LC, Hollingshead, MG (1997) 2-Chloroethyl-3-sarcosinam ide-1-nitrosourea, a novel chloroethyl nitrosourea analogue with enhanced antitumor activity against human glioma xenografs. Cancer Res. 57: pp. 3895-3898
- Skapek, SX, Vandellen, AF, McMahon, DP (1991) Melphalaninduced toxicity in nude mice following pretreatment with buthionine sulfoximine. Cancer Chem Pharmacol. 28: pp. 15-21 CrossRef
- Sato etol. Eradicate cancer. Taipei: ShiMao publisher. 1997; 226–231.
- Burynski, SR (1986) Antineoplastons: History of the research (1). Drugs Exp Clin Res. 12: pp. 1-9
- Feng, PY, Li, Q, Wang, ZJ (2002) The clinic study of CDA-H improve life quality of terminal malignancy patient. China Cancer. 11: pp. 108-200
- Burzynski, SR, Lewy, RI, Weaver, RA (2003) Phase II study of antineoplaston A10 and AS2-1 in patients with recurrent diffuse intrinsic brain stem glioma: a preliminary report. Drugs R&D. 4: pp. 91-101 CrossRef
- Liu, JW, Tang, Y, Shen, Y (2003) Antitumor effect of arsenic trioxide in combination with uroacitides on hepatocellular carcinoma. Chin J Gen Surg. 18: pp. 114-116
- Liu, JW, Tang, Y, Shen, Y (2003) Apoptosis threshold of hepatoma cell lines induced by As2O3 and CDA-II. Chin J Exp Surg. 20: pp. 116-117
- Liu, JW, Tang, Y, Shen, Y (2003) Synergistic effect of cell differentiation agent II and arsenic trioxide on induction of cell cycle and apoptosis in hepatotumor cells. World J Gastroentero 9: pp. 65-68
- Lin, WC, Wu, YW, Lai, TY (2001) Effect of CDA-II, urinary preparation, on lipofuscin, lipid peroxidation and antioxidant systems in young and middle-aged rat brain. Am J Chin Med. 29: pp. 91-99 CrossRef
- Natarajan, V, Scribner, WM, Al-Hassani, M (1998) Reactive oxygen species signaling through regulation of protein tyrosine phos-phorylation in endothelial cells. Environ Health Perspect. 106: pp. 1205-1212 CrossRef
- Anti-tumor effect of CDA-II on a human glioma cell
Chinese Journal of Clinical Oncology
Volume 2, Issue 3 , pp 679-684
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- anti-tumor effect
- induced differentiation