Endocrine Pathology

, Volume 6, Issue 4, pp 279–287

Immunohistochemical analysis of the cell cycle-associated antigens Ki-67 and retinoblastoma protein in parathyroid carcinomas and adenomas

Authors

  • Ricardo V. Lloyd
    • Department of Laboratory Medicine and PathologyMayo Clinic and Mayo Foundation
    • Department of SurgeryMayo Clinic and Mayo Foundation
    • Department of Biostatistics, Mayo ClinicMayo Clinic and Mayo Foundation
  • J. Aidan Carney
    • Department of Laboratory Medicine and PathologyMayo Clinic and Mayo Foundation
    • Department of SurgeryMayo Clinic and Mayo Foundation
    • Department of Biostatistics, Mayo ClinicMayo Clinic and Mayo Foundation
  • Jorge A. Ferreiro
    • Department of Laboratory Medicine and PathologyMayo Clinic and Mayo Foundation
    • Department of SurgeryMayo Clinic and Mayo Foundation
    • Department of Biostatistics, Mayo ClinicMayo Clinic and Mayo Foundation
  • Long Jin
    • Department of Laboratory Medicine and PathologyMayo Clinic and Mayo Foundation
    • Department of SurgeryMayo Clinic and Mayo Foundation
    • Department of Biostatistics, Mayo ClinicMayo Clinic and Mayo Foundation
  • Geoffrey B. Thompson
    • Department of Laboratory Medicine and PathologyMayo Clinic and Mayo Foundation
    • Department of SurgeryMayo Clinic and Mayo Foundation
    • Department of Biostatistics, Mayo ClinicMayo Clinic and Mayo Foundation
  • Jon A. van Heerden
    • Department of Laboratory Medicine and PathologyMayo Clinic and Mayo Foundation
    • Department of SurgeryMayo Clinic and Mayo Foundation
    • Department of Biostatistics, Mayo ClinicMayo Clinic and Mayo Foundation
  • Clive S. Grant
    • Department of Laboratory Medicine and PathologyMayo Clinic and Mayo Foundation
    • Department of SurgeryMayo Clinic and Mayo Foundation
    • Department of Biostatistics, Mayo ClinicMayo Clinic and Mayo Foundation
  • Peter C. Wollan
    • Department of Laboratory Medicine and PathologyMayo Clinic and Mayo Foundation
    • Department of SurgeryMayo Clinic and Mayo Foundation
    • Department of Biostatistics, Mayo ClinicMayo Clinic and Mayo Foundation
Clinical Research

DOI: 10.1007/BF02738728

Cite this article as:
Lloyd, R.V., Carney, J.A., Ferreiro, J.A. et al. Endocr Pathol (1995) 6: 279. doi:10.1007/BF02738728

Abstract

The morphologic distinction between parathyroid carcinoma and adenoma can be a difficult diagnostic problem. We analyzed nuclear immunoreactivity for the cell cycle-associated antigen Ki-67 with monoclonal antibody (MAb) MIB-1 and for retinoblastoma (RB) protein with two polyclonal antisera in 24 parathyroid carcinomas and 35 adenomas, which were formalin fixed and paraffin embedded to determine if these antibodies could assist in distinguishing between carcinomas and adenomas. In addition, 10 cases of parathyroid hyperplasia and 5 cases of normal parathyroids were examined as control tissues. The Ki-67 labeling index was significantly higher in parathyroid carcinomas compared to adenomas (7.1 ± 1.0% vs 2.4 ± 0.2%,p<0.001). No patient with a parathyroid adenoma, parathyroid hyperplasia, or normal parathyroid gland had a Ki-67 labeling index >5.3%. Analysis of the primary tumors from patients with recurrent carcinomas and from those with nonrecurrent carcinomas showed a higher mean Ki-67 labeling index (7.8 ± 1.5% vs 5.2 ± 1.1%), in the former group, although these differences were not statistically significant. The RB protein immunoreactivity was not useful in distinguishing between parathyroid carcinomas and adenomas in paraffin-tissue sections.

These results indicate that nuclear immunoreactivity for the cell cycle-associated antigen Ki-67 may be another useful method to assist in distinguishing parathyroid carcinomas from adenomas.

Key Words

ParathyroidKi-67retinoblastomacarcinomaadenoma

Copyright information

© Humana Press Inc 1995