Abstract
Oleic acid induces lung injury in the baboon. Within 90 min of intravenous oleic acid injection, there are significant increases relative to baseline in the bronchoalveolar lavage fluid (BALF) contents of total protein, lactic dehydrogenase, hexosaminidase, acid protease, and angiotensin-converting enzyme, indicating increased alveolar permeability. When BALF is chromatographed on Sephadex G-200, the elution profiles of hexosaminidase, acid protease, and angiotensin-converting enzyme in BALF are similar to those from normal plasma. SDS-polyacrylamide gel electrophoresis of BALF proteins indicates a similar molecular weight distribution in baseline and post-oleic-acid BALF. No evidence of serine proteases active in the inflammatory process, and present in many cases of human adult respiratory failure, is found either by incubating BALF with hemoglobin and elastin substrates or by labeling BALF with3H-diisopropylfluorophosphate. However, there is a significant increase in α1-antiprotease activity in BALF relative to the baseline value. The activities in plasma of several acute phase reactants including antiproteases, fibronectin, and angiotensin-converting enzyme do not change in this acute phase of the lung injury. The data indicate that many biochemical markers used to indicate acute respiratory failure in humans do not change acutely in the expected fashion in this animal model of the disease.
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Collins, J.F., de los Santos, R. & Johanson, W.G. Acute effects of oleic acid-induced lung injury in baboons. Lung 164, 259–268 (1986). https://doi.org/10.1007/BF02713650
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DOI: https://doi.org/10.1007/BF02713650