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Acute effects of oleic acid-induced lung injury in baboons

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Abstract

Oleic acid induces lung injury in the baboon. Within 90 min of intravenous oleic acid injection, there are significant increases relative to baseline in the bronchoalveolar lavage fluid (BALF) contents of total protein, lactic dehydrogenase, hexosaminidase, acid protease, and angiotensin-converting enzyme, indicating increased alveolar permeability. When BALF is chromatographed on Sephadex G-200, the elution profiles of hexosaminidase, acid protease, and angiotensin-converting enzyme in BALF are similar to those from normal plasma. SDS-polyacrylamide gel electrophoresis of BALF proteins indicates a similar molecular weight distribution in baseline and post-oleic-acid BALF. No evidence of serine proteases active in the inflammatory process, and present in many cases of human adult respiratory failure, is found either by incubating BALF with hemoglobin and elastin substrates or by labeling BALF with3H-diisopropylfluorophosphate. However, there is a significant increase in α1-antiprotease activity in BALF relative to the baseline value. The activities in plasma of several acute phase reactants including antiproteases, fibronectin, and angiotensin-converting enzyme do not change in this acute phase of the lung injury. The data indicate that many biochemical markers used to indicate acute respiratory failure in humans do not change acutely in the expected fashion in this animal model of the disease.

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References

  1. Beatty K, Robertie P, Senior RM, Travis J (1982) Determination of oxidized alpha-1-proteinase inhibitor in serum. J Lab Clin Med 100:186–192

    PubMed  CAS  Google Scholar 

  2. Bedrossian CWM, Woo J, Miller WC, Cannon DC (1978) Decreased angiotensin converting enzyme in the adult respiratory distress syndrome. Am J Clin Pathol 70:244–247

    PubMed  CAS  Google Scholar 

  3. Bell RC, Coalson JJ, Smith JD, Johanson WG Jr (1983) Multiple organ failure and infection in adult respiratory distress syndrome. Ann Intern Med 99:293–298

    PubMed  CAS  Google Scholar 

  4. Campbell GD, Coalson JJ, Johanson WG Jr (1984) The effect of bacterial superinfection on lung function after diffuse alveolar damage. Am Rev Respir Dis 129:974–978

    PubMed  CAS  Google Scholar 

  5. Collins JF, Smith JD, Coalson JJ, Johanson WG Jr (1984) Variability in lung collagen amounts after prolonged support of acute respiratory failure. Chest 85:641–646

    PubMed  CAS  Google Scholar 

  6. Dubois RM, Townsend PJ, Cole PJ (1980) Alveolar macrophage lysosomal enzyme and C3b receptors in crytogenic fibrosing alveolitis. Clin Exp Immunol 40:60–65

    CAS  Google Scholar 

  7. Erlanger BF, Kokowsky N, Cohen W (1961) The preparation and properties of two new chromogenic substrates of trypsin. Arch Biochem Biophys 95:271–278

    Article  PubMed  CAS  Google Scholar 

  8. Fine R, Campbell ME, Collins JF (1984) Elastolytic activity of peripheral blood monocytes. Connective Tissue Res 13:27–35

    CAS  Google Scholar 

  9. Fine R, Collins JF (1982) The effect of theophylline on macrophage elastase secretion. Connective Tissue Res 9:195–199

    Article  CAS  Google Scholar 

  10. Fowler AA, Hamman RF, Good JT, Benson KN, Baird M, Eberle D, Petty TL, Hyers TM (1983) The adult respiratory distress syndrome: risk with common predispositions. Ann Intern Med 98:593–597

    PubMed  CAS  Google Scholar 

  11. Fowler AA, Hamman RF, Zerbe GO, Benson KN, Hyers TM (1985) Adult respiratory distress syndrome: prognosis after onset. Am Rev Respir Dis 132:472–478

    PubMed  CAS  Google Scholar 

  12. Fowler AA, Walchak S, Giclas PC, Henson PH, Hyers TM (1982) Characterization of antiproteinase activity in the adult respiratory distress syndrome. Chest 81:50S-51S

    Article  Google Scholar 

  13. Gadek JE, Kelman JA, Fells G, Weinberger SE, Horowitz AL, Reynolds HY, Fulmer JD, Crystal RG (1979) Collagenase in the lower respiratory tract of patients with idiopathic pulmonary fibrosis. N Engl J Med 301:737–742

    Article  PubMed  CAS  Google Scholar 

  14. Grossman J, Will L, Hahn N, Exten R, Garber C, Mosher D (1983) Plasma fibronectin in critical illness. Am Rev Respir Dis 127:95 (abstr)

    Google Scholar 

  15. Horowitz AL, Kelman JA, Crystal RG (1976) Activation of alveolar macrophage collagenase by a neutral protease secreted by the same cell. Nature (London) 264:772–774

    Article  Google Scholar 

  16. Johanson WG Jr, Holcomb JR, Coalson JJ (1982) Experimental diffuse alveolar damage in baboons. Am Rev Respir Dis 126:142–151

    PubMed  Google Scholar 

  17. Labrousse J, Couland JM, Jacqueson A, Rapin J, Beyne P, Salmona JF, Tenaillon A, Lissac J (1982) Plasma fibronectin concentration in acute respiratory failure. Am Rev Respir Dis 125:132 (abstr)

    Google Scholar 

  18. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685

    Article  CAS  Google Scholar 

  19. Lee CT, Fein AM, Lippmann M, Holtzman H, Kimbel P, Weinbaum G (1981) Elastolytic activity in pulmonary lavage fluid from patients with adult respiratory distress syndrome. N Engl J Med 304:192–196

    Article  PubMed  CAS  Google Scholar 

  20. Lieberman J (1975) Elevation of serum angiotensin-converting enzyme in sarcoidosis. 59:365–372

    CAS  Google Scholar 

  21. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folinphenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  22. Maunder RJ, Harlan JM, Pepe PE, Holtman Revs D, Hudson LD, Carrico CJ (1983) Fibonectin levels in patients developing ARDS. Am Rev Respir Dis 127:95 (abstr)

    Google Scholar 

  23. McCullough B, Schneider S, Greene ND, Johanson WG, Jr (1978) Bleomycin-induced lung injury in baboons: alteration of cells and immunoglobins recoverable by bronchoalveolar lavage. Lung 155:337–358

    PubMed  CAS  Google Scholar 

  24. McGuire WM, Spragg RG, Cohen AB, Cochrane CG (1982) Studies on the pathogenesis of the adult respiratory distress syndrome. J Clin Invest 69:543–553

    PubMed  CAS  Google Scholar 

  25. Miles LA, Plow EF, Donnelly KJ, Hougie C, Griffin JH (1982) A bleeding disorder due to deficiency ofα 2-antiplasmin. Blood 59:1246–1251

    PubMed  CAS  Google Scholar 

  26. Montgomery AB, Stager MA, Carrico CJ, Hudson LD (1985) Causes of mortality in patients with the adult respiratory distress syndrome. Am Rev Respir Dis 132:485–489

    PubMed  CAS  Google Scholar 

  27. Nerlich AG, Nerlich ML, Joachim H, Muller PK (1984) Biochemical changes in parenchymal lung tissue associated with posttraumatic respiratory failure. Eur J Clin Invest 14:190–198

    Article  PubMed  CAS  Google Scholar 

  28. Newman RA, Kimberly PJ, Stewart JA, Kelley J (1980) Assessment of bleomycin lung toxicity using angiotensin-converting enzyme in pulmonary lavage. Cancer Res 40:3621–3626

    PubMed  CAS  Google Scholar 

  29. Petty TL, Ashbaugh DG (1971) The adult respiratory distress syndrome: clinical features and factors influencing prognosis and principles of management. Chest 70:233–239

    Google Scholar 

  30. Shaw JO, Ferrigni KS, Wetsel RA, Andrews CP, Collins JF, Fowler SR, Kolb WP (1986) Enzymes capable of cleaving the fifth component of complement in bronchoalveolar lavage fluid from patients with adult respiratory distress syndrome: Generation of chemotactic activity in airspaces. Submitted.

  31. Thoman PJ, Shaw SL, Collins JF (1985) Isolation and characterization of fibronectin from baboon plasma. Connective Tissue Res 13:135–143

    CAS  Google Scholar 

  32. Woollen JW, Heyworth R, Walker PG (1961) Studies on glucosaminidase. 3. Testicular N-acetyl-beta-galactosaminidase. Biochem J 78:111–116

    PubMed  CAS  Google Scholar 

  33. Zapol WM, Trelstad RL, Coffey JW, Tsai I, Salvador RA (1979) Pulmonary fibrosis in acute respiratory failure. Am Rev Respir Dis 119:457–454

    Google Scholar 

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Collins, J.F., de los Santos, R. & Johanson, W.G. Acute effects of oleic acid-induced lung injury in baboons. Lung 164, 259–268 (1986). https://doi.org/10.1007/BF02713650

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