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Determination of acetylcholine and choline in the femtomole range by means of HPLC, a post-column enzyme reactor, and electrochemical detection

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Summary

The measurement of choline and acetylcholine by means of HPLC, a post-column enzyme reactor, and electrochemical detection has been simplified and optimised. The use of a cation exchanger and enzyme reactor fitted in a cartridge holder appeared to result in reproducible, sensitive, and selective measurement of endogenous choline and acetylcholine with a lower detection limit of 50 fmole.

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Authors and Affiliations

  1. Department of Medicinal Chemistry, University of Groningen, A. Deusinglaan 2, 9713 AW, Groningen, The Netherlands

    G. Damsma, B. H. C. Westerink & A. S. Horn

  2. Chrompack International, Middelburg, The Netherlands

    D. Lammerts van Bueren

Authors
  1. G. Damsma
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  2. D. Lammerts van Bueren
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  3. B. H. C. Westerink
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  4. A. S. Horn
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Damsma, G., van Bueren, D.L., Westerink, B.H.C. et al. Determination of acetylcholine and choline in the femtomole range by means of HPLC, a post-column enzyme reactor, and electrochemical detection. Chromatographia 24, 827–831 (1987). https://doi.org/10.1007/BF02688592

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  • DOI: https://doi.org/10.1007/BF02688592

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