, Volume 24, Issue 1, pp 400–402

A new polymer supported packing material as a substitute of hydroxylapatite: Purification of Topoisomerases

  • K. Majumder
  • N. Ramesh

DOI: 10.1007/BF02688514

Cite this article as:
Majumder, K. & Ramesh, N. Chromatographia (1987) 24: 400. doi:10.1007/BF02688514


Hydroxylapatite (HA), a form of calcium phosphate, finds extensive usages in the fractionation and purification of proteins, enzymes and nucleic acids. However, commercial HA preparations for liquid chromatography (LC) differ from many other LC packing materials in that the former is not polymer supported. We have now developed a substitute packing material which uses Fractosil 200 as the polymer support. Other polymer supports can also be similarly used. Being Fractosil 200 supported, this Hydroxylapatite Substitute (HAS) matrix can be employed for open column LC as well as for HPLC purposes. Although the exact chemical nature of HAS is different from the conventional HA, HAS mimicks the functions of the latter support. Due to the high’ binding capacity of HA many DNA binding proteins have been partially purified on this support. In the present work HAS has been successfully used in the partial purification of Topoisomerase I and II from wheat germ.

Key Words

Liquid chromatographyHydroxylapatite substitute, HASProtein purificationTopoisomerases

List of Common Abbreviations




Bovine serum albumin


Deoxyribonucleic acid




Ethylenediaminetetraacetic acid


40 mM Tris-acetate, 2 mM EDTA


Tris(hydroxymethyl) aminomethane

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • K. Majumder
    • 1
  • N. Ramesh
    • 1
  1. 1.Molecular Biophysics UnitIndian Institute of ScienceBangaloreIndia