Genetic Resource

Plant Molecular Biology Reporter

, Volume 8, Issue 1, pp 40-49

Medicago truncatula, a model plant for studying the molecular genetics of theRhizobium-legume symbiosis

  • David G. BarkerAffiliated withLaboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS
  • , Sylvie BianchiAffiliated withLaboratoire d'Amélioration des Plantes, Université de Paris-Sud
  • , François BlondonAffiliated withLaboratoire du Phytotron, CNRS
  • , Yvette DattéeAffiliated withLaboratoire d'Amélioration des Plantes, Université de Paris-Sud
  • , Gérard DucAffiliated withStation de Génétique et d'Amélioration des Plantes, INRA, BV 1540
  • , Sadi EssadAffiliated withStation de Génétique et d'Amélioration des Plantes, INRA
  • , Pascal FlamentAffiliated withLaboratoire d'Amélioration des Plantes, Université de Paris-Sud
  • , Philippe GallusciAffiliated withLaboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS
  • , Gérard GénierAffiliated withStation d'Amélioration des Plantes Fourragéres, INRA
    • , Pierre GuyAffiliated withStation d'Amélioration des Plantes Fourragéres, INRA
    • , Xavier MuelAffiliated withLaboratoire de Biologie Cellulaire, INRA
    • , Jacques TourneurAffiliated withLaboratoire de Biologie Cellulaire, INRA
    • , Jean DénariéAffiliated withLaboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS
    • , Thierry HuguetAffiliated withLaboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS

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Abstract

Medicago truncatula has all the characteristics required for a concerted analysis of nitrogen-fixing symbiosis withRhizobium using the tools of molecular biology, cellular biology and genetics.M. truncatula is a diploid and autogamous plant has a relatively small genome, and preliminary molecular analysis suggests that allelic heterozygosity is minimal compared with the cross-fertilising tetraploid alfalfa (Medicago sativa). TheM. truncatula cultivar Jemalong is nodulated by theRhizobium meliloti strain 2011, which has already served to define many of the bacterial genes involved in symbiosis with alfalfa. A genotype of Jemalong has been identified which can be regenerated after transformation byAgrobacterium, thus allowing the analysis ofin-vitro-modified genes in an homologous transgenic system. Finally, by virtue of the diploid, self-fertilising and genetically homogeneous character ofM. truncatula, it should be relatively straightforward to screen for recessive mutations in symbiotic genes, to carry out genetic analysis, and to construct an RFLP map for this plant.

Keywords

symbiotic nitrogen fixation legume Medicago truncatula