Plant Molecular Biology Reporter

, Volume 8, Issue 1, pp 40–49

Medicago truncatula, a model plant for studying the molecular genetics of theRhizobium-legume symbiosis

Authors

  • David G. Barker
    • Laboratoire de Biologie Moléculaire des Relations Plantes-MicroorganismesINRA-CNRS
  • Sylvie Bianchi
    • Laboratoire d'Amélioration des PlantesUniversité de Paris-Sud
  • François Blondon
    • Laboratoire du PhytotronCNRS
  • Yvette Dattée
    • Laboratoire d'Amélioration des PlantesUniversité de Paris-Sud
  • Gérard Duc
    • Station de Génétique et d'Amélioration des PlantesINRA, BV 1540
  • Sadi Essad
    • Station de Génétique et d'Amélioration des PlantesINRA
  • Pascal Flament
    • Laboratoire d'Amélioration des PlantesUniversité de Paris-Sud
  • Philippe Gallusci
    • Laboratoire de Biologie Moléculaire des Relations Plantes-MicroorganismesINRA-CNRS
  • Gérard Génier
    • Station d'Amélioration des Plantes FourragéresINRA
  • Pierre Guy
    • Station d'Amélioration des Plantes FourragéresINRA
  • Xavier Muel
    • Laboratoire de Biologie CellulaireINRA
  • Jacques Tourneur
    • Laboratoire de Biologie CellulaireINRA
  • Jean Dénarié
    • Laboratoire de Biologie Moléculaire des Relations Plantes-MicroorganismesINRA-CNRS
  • Thierry Huguet
    • Laboratoire de Biologie Moléculaire des Relations Plantes-MicroorganismesINRA-CNRS
Genetic Resource

DOI: 10.1007/BF02668879

Cite this article as:
Barker, D.G., Bianchi, S., Blondon, F. et al. Plant Mol Biol Rep (1990) 8: 40. doi:10.1007/BF02668879

Abstract

Medicago truncatula has all the characteristics required for a concerted analysis of nitrogen-fixing symbiosis withRhizobium using the tools of molecular biology, cellular biology and genetics.M. truncatula is a diploid and autogamous plant has a relatively small genome, and preliminary molecular analysis suggests that allelic heterozygosity is minimal compared with the cross-fertilising tetraploid alfalfa (Medicago sativa). TheM. truncatula cultivar Jemalong is nodulated by theRhizobium meliloti strain 2011, which has already served to define many of the bacterial genes involved in symbiosis with alfalfa. A genotype of Jemalong has been identified which can be regenerated after transformation byAgrobacterium, thus allowing the analysis ofin-vitro-modified genes in an homologous transgenic system. Finally, by virtue of the diploid, self-fertilising and genetically homogeneous character ofM. truncatula, it should be relatively straightforward to screen for recessive mutations in symbiotic genes, to carry out genetic analysis, and to construct an RFLP map for this plant.

Keywords

symbiotic nitrogen fixationlegumeMedicago truncatula

Abbreviations

RFLP

restriction fragment length polymorphism

Copyright information

© Kluwer Academic Publishers 1990