Skip to main content
Log in

Analysis of soybean lecithin by thin layer and analytical liquid chromatography

  • Technical
  • Published:
Journal of the American Oil Chemists Society

Abstract

The application of thin layer and analytical liquid chromatography to the analysis of two samples of commercial soybean lecithins is described. A combination of column chromatography and quantitative thin layer chromatography showed that these products consisted of ca. 82% mixture of the major phospholipids of soybeans, phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidyl inositol. The remainder of these products contained essentially the entire spectrum of lipid classes found in soybean oil-some 24 known and unknown glycolipids and phospholipids, in addition to the neutral lipids. Applications of analytical liquid chromatography to these lecithins gave a composition profile of the lipid classes comparable to two-dimensional thin layer chromatography. The potential of this method for the complete analysis of complex lipids, such as soybean lecithins, is indicated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Food Chemicals Codex National Academy of Science, National Research Council Publication 1143:599 (1962). Official and Tentative Methods of Analysis of the American Oil Chemists' Society, Section J.

    Google Scholar 

  2. Szuhaj, B.F., Balazs, I.L. and L.D. Williams, Paper 175, World Congress, American Oil Chemists' Society and International Society for Fat Research, Chicago, Ill., September 1970.

  3. Rouser, R., G. Kritchevsky and A. Yamamoto, in “Lipid Chromatographic Analysis,” Vol. 1, Edited by G.V. Marinetti, Marcel Dekker, Inc., New York, N.Y., 1967, pp. 99–161.

    Google Scholar 

  4. Lepage, M., J. Chromatogr. 13:99 (1964).

    Article  CAS  Google Scholar 

  5. Privett, O.S., K.A. Dougherty and J.D. Castell, Am. J. Clin. Nutr. 24:1265 (1971).

    CAS  Google Scholar 

  6. Renkonen, O., and P. Varo, in “Lipid Chromatographic Analysis,” Vol. 1, Edited by G.V. Marinetti, Marcel Dekker, Inc., New York, N.Y., 1967, p. 41.

    Google Scholar 

  7. Dittmer, S.C., and R.L. Lester, J. Lipid Res. 5:126 (1964).

    CAS  Google Scholar 

  8. Skipski, V.P., and M. Barclay, in “Methods of Enzymology,” Edited by J.M. Lowenstein, Academic Press, New York, N.Y., 1969, p. 542.

    Google Scholar 

  9. Blank, M.L., J.A. Schmit and O.S. Privett, JAOCS 41:371 (1964).

    CAS  Google Scholar 

  10. Stolyhwo, A., and O.S. Privett, J. Chromatogr. Sci. 11:20 (1973).

    CAS  Google Scholar 

  11. Stolyhwo, A., W.L. Erdahl and O.S. Privett, J. Chromatogr. Sci. 11:263 (1973).

    CAS  Google Scholar 

  12. Privett, O.S., K.A. Dougherty, W.L. Erdahl and A. Stolyhwo, JAOCS 50:516 (1973).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

About this article

Cite this article

Erdahl, W.L., Stolyhwo, A. & Privett, O.S. Analysis of soybean lecithin by thin layer and analytical liquid chromatography. J Am Oil Chem Soc 50, 513–515 (1973). https://doi.org/10.1007/BF02640522

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02640522

Keywords

Navigation