Summary
Low density bovine vacular endothelial cell cultures maintained on dishes coated with an extracellular matrix can be grown in serum-free Dulbecco's modified Eagle's medium supplemented with high density lipoprotein (HDL) and transferrin. Such cultures do not require insulin. Early passage cultures exposed to HDL and transferrin grew as well as cultures exposed to optimal serum concentrations and could be passaged repeatedly in total absence of serum. A requirement for fibroblast growth factor to ensure an optimal growth could be observed only with late-passage cultures. The present results suggest strongly that HDL is involved in supporting the proliferation of vascular endothelial cells in vitro. This may be important for our understanding of the biological role of HDL “in vivo”.
Similar content being viewed by others
References
Gospodarowicz, D.; Ill, C. R. The extracellular matrix and the control of proliferation of vascular endothelial cells. J. Clin. Invest. 65: 1351–1364; 1980.
Gospodarowicz, D.; Savion, N.; Vlodavsky, I. The extracellular matrix and the control of proliferation of vascular endothelial and vascular smooth muscle cells. J. Supramolecular Struct. 13: 339–372; 1980.
Gospodarowicz, D.; Delgado, D.; Vlodavsky, I. Permissive effect of the extracellular matrix on cell proliferation in vitro. Proc. Natl. Acad. Sci. USA 77: 4094–4098; 1980.
Miller, G. J.; Miller, N. E. Plasma-high-density-lipoprotein concentration and development of ischemic heart disease. Lancet 1: 16–19; 1975.
Tauber, J.-P.; Cheng, J.; Gospodarowicz, D. The effect of high and low density lipoproteins on the proliferation of vascular endothelial cells maintained in the presence of lipoprotein-deficient serum. J. Clin. Invest. 66: 696–708; 1980.
Gospodarowicz, D.; Bialecki, H.; Greenburg, G. Purification of the fibroblast growth factor activity from bovine brain. J. Biol. Chem. 253: 3736–3743; 1978.
Havel, R. J.; Eder, H. S.; Bragdon, J. A. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J. Clin. Invest. 34: 1345–1353; 1955.
Jonas, A. Physicochemical properties of bovine serum high density lipoproteins. J. Biol. Chem. 247: 7767–7772; 1972.
Gospodarowicz, D.; Greenburg, G. The coating of bovine and rabbit corneas denuded of their endothelium with bovine corneal endothelial cells. Exp. Eye Res. 38: 249–265; 1978.
Gospodarowicz, D.; Mescher, A. L.; Birdwell, C. R. Stimulation of corneal endothelial cells in vitro by fibroblast and epidermal growth factors. Exp. Eye Res. 25: 75–87; 1977.
Gospodarowicz, D.; Hirabayashi, K.; Giguere, L.; Tauber, J.-P. Factors controlling the proliferative rate, final cell density, and life span of bovine vascular smooth muscle cells in culture. J. Cell Biol. In press.
Gospodarowicz, D.; Greenburg, G.; Bialecki, H.; Zetter, B. Factors involved in the modulation of cell proliferation in vivo and in vitro: The role of fibroblast and epidermal growth factors in the proliferative response of mammalian cells. In Vitro 14: 85–118; 1978.
Vlodavsky, I.; Johnson, L. K.; Greenburg, G.; Gospodarowicz, D. Vascular endothelial cells maintained in the absence of fibroblast growth factor undergo structural and functional alterations that are incompatible with their in vivo differentiated properties. J. Cell Biol. 83: 468–483; 1979.
Phillips, J. L. Specific binding of radioiodinated transferrin to polypropylene culture tubes. Biochem. Biophys. Res. Commun. 71: 726–732; 1976.
Barnes, D.; Sato, G. Serum-free cell culture: a unifying approach. Cell 22: 649–655; 1980.
Bottenstein, J.; Hayashi, I.; Hutchings, S.; Masui, H.; Mather, J.; McClure, D.; Ohasa, S.; Rizzino, A.; Sato, G.; Serrero, G.; Wolfe, R.; Wu, R. The growth of cells in serum-free hormone-supplemented medium. Methods Enzymol. 58: 94–109; 1978.
Sato, G. The role of serum in cell culture. Litwak, G. ed. Biochemical action of hormones. Vol. 3. New York: Academic Press; 1975: 391–396.
Sato, G. H.; Reid, L. Replacement of serum in cell culture by hormones. Biochemistry and mode of action of hormones II. Rickenberg, H. V. ed. Vol. 20. Baltimore, MD: University Park; 1978: 215–256.
Hutchings, S. E.; Sato, G. H. Growth and maintenance of Hela cells in serum-free medium supplemented with hormones. Proc. Natl. Acad. Sci. USA 75: 901–904; 1978.
Rizzino, A.; Sato, G. Growth of embryonal carcinoma cells in serum-free medium. Proc. Natl. Acad. Sci. USA 75: 1844–1848; 1978.
Gospodarowicz, D.; Fujii, D. K. The extracellular matrix and the control of cell proliferation and differentiation. Scott, W. A.; Werner, R.; Schultz, J. eds. Cellular responses to molecular modulators. Miami Winter Symposia, Vol. 18. In press.
Savion, N.; Liu, G. M.; Laherty, R.; Gospodarowicz, D. Factors controlling the proliferation and progesterone production by bovine granulosa cells in serum free medium. Endocrinology. In press.
Author information
Authors and Affiliations
Additional information
This work was supported by Grants HL 23678 and 20192 from the National Institutes of Health, Bethesda, MD.
Rights and permissions
About this article
Cite this article
Tauber, JP.P., Cheng, J., Massoglia, S. et al. High density lipoproteins and the growth of vascular endothelial cells in serum-free medium. In Vitro 17, 519–530 (1981). https://doi.org/10.1007/BF02633513
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02633513