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The effect of tritiated thymidine on human chromosomes

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The effect of low dosages of3H-thymidine on human chromosomes was investigated. Two established human cell lines, amnion and HeLa cells, were treated for various periods of time 0.125, 0.25, 0.50 or 0.75 μCi/ml of this agent. Chromatid-type aberrations were observed, occurring only if3H-thymidine was incorporated into DNA during the S phase of the cell cycle. The higher the dosage and the longer the3H-thymidine acted on chromatin, the greater the number of aberrations induced. Chromosomes were most sensitive to the effects of3H-thymidine during the early S phase of the cell cycle.

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This work was supported by the Medical Research Council, Canada Grant MA 1639.

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Wang, H.C., Fedoroff, S. The effect of tritiated thymidine on human chromosomes. In Vitro 7, 74–79 (1971). https://doi.org/10.1007/BF02628265

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